West China Journal of Stomatology ›› 2018, Vol. 36 ›› Issue (1): 17-22.doi: 10.7518/hxkq.2018.01.004

• Orginal Article • Previous Articles     Next Articles

Preparation and characterization of oriented scaffolds derived from cartilage extracellular matrix and silk fibroin

Binhong Teng1(), Yanhong Zhao1(), Lianyong Wang2, Qiang Yang3, Hongfa Li1, Yunjie Li1   

  1. 1. Dept. of Orthodontics, Stomato-logical Hospital of Tianjin Medical University, Tianjin 300070, China
    2. The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China
    3. Dept. of Spine Surgery, Tianjin Hospital, Tianjin 300211, China
  • Received:2017-05-05 Revised:2017-09-02 Online:2018-02-07 Published:2018-02-01
  • Supported by:
    Supported by: The National Natural Science Foundation of China (31300798, 31470937, 81572154);The Key Project of Science and Technology Research of Tianjin Health Bureau (15KG125, 16KG114).


Objective This study aims to prepare oriented scaffolds derived from a cartilage extracellular matrix (CECM) and silk fibroin (SF) and use to investigate their physicochemical property in cartilage tissue engineering. Methods Oriented SF-CECM scaffolds were prepared from 6% mixed slurry (CECM:SF=1:1) through modified temperature gradient-guided thermal-induced phase separation, followed by freeze drying. The SF-CECM scaffolds were evaluated by scanning electron microscopy (SEM) and histological staining analyses and determination of porosity, water absorption, and compressive elastic modulus of the materials. Results The SEM image showed that the SF-CECM scaffolds contained homogeneous reticular porous structures in the cross-section and vertical tubular structures in the longitudinal sections. Histological staining showed that cells were completely removed, and the hybrid scaffolds retained proteogly can and collagen. The composition of the scaffold was similar to that of natural cartilage. The porosity, water absorption rate, and vertical compressive elastic modulus of the scaffolds were 95.733%±1.010%, 94.309%±1.302%, and (65.40±4.09) kPa, respectively. Conclusion The fabricated SF-CECM scaffolds exhibit satisfactory physicochemical and biomechanical properties and thus could be an ideal scaffold in cartilage tissue engineering.

Key words: cartilage, silk fibroin, extracellular ma-trix, oriented scaffolds, tissue engineering

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