West China Journal of Stomatology ›› 2019, Vol. 37 ›› Issue (3): 253-259.doi: 10.7518/hxkq.2019.03.005

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Preparation and evaluation of carboxymethyl chitosan/sodium alginate hydrogel for cartilage tissue engineering

Yunjie Li1,Binhong Teng1,Yanhong Zhao1(),Qiang Yang2,Lianyong Wang3,Ying Huang1   

  1. 1.Dept. of Orthodontics, Stomatological Hospital of Tianjin Medical University, Tianjin 300070, China
    2.Dept. of Spine Surgery, Tianjin Hospital, Tianjin 300211, China
    3.The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China
  • Received:2018-12-15 Revised:2019-03-10 Online:2019-06-01 Published:2019-06-12
  • Contact: Yanhong Zhao E-mail:leafzh@126.com
  • Supported by:
    The National Science Foundation of China(31300798);The National Science Foundation of China(31470937);The National Science Foundation of China(81572154);The Research Foundation of Tianjin Health Bureau(15KG125);The Research Foundation of Tianjin Health Bureau(16KG114)


Objective This study aimed to optimize the preparation of carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels. This study also aimed to investigate the applicability of the hydrogels in cartilage tissue engineering. Methods Three groups of CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2:1, 1:1 and 1:2 were prepared. The microstructure, physical properties, and cell biocompatibility of the three groups of CMCS/OSA composite hydrogels were evaluated. Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering. Results The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1:1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility. Conclusion The CMCS/OSA compound hydrogel prepared with a 1:1 ratio of amino and aldehyde groups has potential applications in cartilage tissue engineering.

Key words: carboxymethyl chitosan, sodium alginate, hydrogel, umbilical cord mesenchymalstem cell, cartilage tissue engineering

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