Basic fibroblast growth factor/chitosan derivatives/collagen composite thermosensitive hydrogel promotes perio-dontal tissue regeneration in rats

doi:10.7518/hxkq.2023.01.003

Abstract

Abstract:

Objective To investigate the feasibility of different thermosensitive composite hydrogels from chitosan derivatives as scaffold materials for periodontal tissue engineering. Methods Three chitosan derivatives with different biological characteristics were prepared, namely, sulfonated chitosan (SCS), phosphorylated chitosan (PCS), and phosphorylated sulfonated chitosan (PSCS). Three thermosensitive composite hydrogels were constructed using basic fibroblast growth factor (bFGF), the chitosan derivatives, and collagen. Twenty male Wistar rats were randomly divided into control group, blank group, bFGF/SCS/collagen composite thermosensitive hydrogel group, bFGF/PCS/collagen compo-site thermosensitive hydrogel group, and bFGF/PSCS/collagen composite thermosensitive hydrogel group. Then, three-wall intrabony defects were established. The defects were treated with the different kinds of thermosensitive composite hydrogels. After 6 weeks of surgery, the animals were killed, and specimens were collected. Then, gross observation, hematoxylin-eosin staining, and Masson staining were performed. Results The bFGF/chitosan derivatives/collagen composite thermosensitive hydrogel groups and the control group had statistical differences in the relative alveolar bone height, relative epithelial down growth and grading count score of periodontal tissue regeneration (P<0.05). Conclusion bFGF/chitosan derivatives/collagen composite thermosensitive hydrogels have good application prospects in periodontal tissue engineering.

Key words: hydrogel, chitosan derivatives, basic fibroblast growth factor, collagen, periodontal tissue, regeneration

CLC Number:

R 781.4

Tan Zhongjuan, Luo Yuanyuan, Yang Li. Basic fibroblast growth factor/chitosan derivatives/collagen composite thermosensitive hydrogel promotes perio-dontal tissue regeneration in rats[J]. West China Journal of Stomatology, 2023, 41(1): 21-28.

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级别	描述
0	没有PDL的再生及CT附着，纤维无序，上皮根向下移
1	没有PDL再生，但有CT附着及垂直方向的纤维形成
2	在缺损上方有PDL再生及垂直方向的纤维形成
3	完全的PDL再生

样品	元素分析					磺化率	磷酸化率
样品	C	H	N	S	P	磺化率	磷酸化率
CS	40.82	7.22	7.35	-	-	-	-
SCS	20.39	4.12	3.99	12.66	-	138.82	-
PCS	32.15	6.56	5.78	-	5.69	-	44.33
PSCS	24	5.27	4.37	12.15	0.34	121.64	3.5

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