Preparation of functional poly-(lactic acid-co-glycolic acid)-based guided bone-regeneration membrane and its application in the reconstruction of mandibular defects in rats

doi:10.7518/hxkq.2022.05.004

Abstract

Abstract:

Objective This study aimed to prepare functional composite electrospinning fibrous membranes with the functions of antifibrosis and bacteriostasis, as well as to explore its repair effect on rat jaw defect. Methods Poly-(lactic acid-co-glycolic acid) fibrous membrane loaded with cyclic-arginine-glycine-aspartic acid sequence, ornidazole, and nanohydroxyapatite (n-HA) was prepared by electrospinning as the functional layer (GBRL) for adhering onto defective bone. A barrier layer with the function of supporting and isolating different functional layers was prepared by tape-casting method. Poly(p-dioxanone-co-l-phenylalanine) fiber membrane with the function of inhibiting fibrosis was prepared by electrospinning technology as the antifibrosis layer (AFL). The morphology of the composite membrane was characterized by scanning electron microscopy. The effects of different functional layers on the proliferation of mouse osteoblast precursor cells (MC3T3-E1) and mouse fibroblasts (L929) were studied by cell-proliferation test (CCK-8 method). The inhibitory effect of composite membrane on the proliferation of Porphyromonas gingivalis was studied by bacteriostatic circle test. A rat mandibular-bone defect model was established to study the repair effect of composite fiber membrane on bone-defect tissue. This repair effect was compared with that of collagen oral-repair membrane commonly used in clinics. Results The GBRL layer could support the proliferation of MC3T3-E1 cells, and the AFL layer could inhibit the proliferation of L929 cells. Composite membranes loaded with ornidazole could inhibit the proliferation of Porphyromonas gingivalis. Various composite membranes can induce the reconstruction of rat jaw defects, among which the composite membranes loaded with ornidazole and n-HA had the best repair effect, which was better than that of collagen oral-repair membrane. Conclusion The electrospun membrane loaded with ornidazole and n-HA as the composite fiber membrane of GBRL layer had excellent antibacterial and bone-tissue-regeneration activity. The effect was better than that of the commonly used collagen oral-repair membrane. Therefore, this material has great potential to induce bone regeneration for defects caused by periodontal diseases.

Key words: electrospinning, anti-fibrosis, bacteriostatic, guided bone regeneration

CLC Number:

R 782.2

Liu Yiming, Zhao Yun, Han Mei, Zhang Yuqiu, Mi Fanglin, Wang Bing. Preparation of functional poly-(lactic acid-co-glycolic acid)-based guided bone-regeneration membrane and its application in the reconstruction of mandibular defects in rats[J]. West China Journal of Stomatology, 2022, 40(5): 522-531.

Figures/Tables 7

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名称	GBRL层	BL层	AFL层
M1	cRGD、n-HA-g-PPDO、PLGA/PLCA静电纺丝膜	-	PDPA/PLGA/PLCA流延膜
M2	cRGD、n-HA-g-PPDO、PLGA/PLCA静电纺丝膜	PLGA/PLCA流延膜	PDPA/PLGA静电纺丝膜
M3	cRGD、n-HA-g-PPDO、奥硝唑、PLGA/PLCA静电纺丝膜	PLGA/PLCA流延膜	PDPA/PLGA静电纺丝膜
M4	cRGD、PPDO、奥硝唑、PLGA/PLCA静电纺丝膜	PLGA/PLCA流延膜	PDPA/PLGA静电纺丝膜

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