Abstract:

Objective  To study the microstructure of the Ag-nHA-nTiO2/PA66 membrane and investigate its biocompatibility. Methods  The microstructure of Ag-nHA-nTiO2/PA66 membrane and e-polytetra fluoroethylene (e-PTFE) membrane were observed by light microscope and scanning electron microscope (SEM) . MG63 osteoblast-like cells were cultured on the two kinds of membrane and blank group. The cell proliferation was checked by methyl thiazolyl te- trazolium (MTT) method and alkaline phosphatase (ALP) activity was detected by enzyme linked immunosorbent assay (ELISA) . The adhesion and proliferation of the cells on the two kinds of membrane was observed by SEM. Results  The Ag-nHA-nTiO2/PA66 membrane was composed of the obverse face and the opposite face. The obverse face was porous and the opposite face was smooth. Microstructures of the obverse and the opposite face of the e-PTFE membrane were same. The e-PTFE membrane showed many tiny lined cracks in elliptic structure. MTT assay and ALP measurement showed that there were no significant difference between each of the two membrane groups and the blank (P>0.05). The adhesion and proliferation of cells on the Ag-nHA-nTiO2/PA66 membrane were better than the e-PTFE membrane. Conclusion  Ag-nHA-nTiO2/PA66 membrane has no negative effects on the growth of osteoblast-like cells. Ag-nHA-nTiO2/PA66 membrane is biocompatible and its microstructure is appropriate as a guided bone regeneration materials.

Key words: guided bone regeneration, hydroxyapatite, silver, MG63 osteoblast-like cells