West China Journal of Stomatology ›› 2020, Vol. 38 ›› Issue (3): 240-244.doi: 10.7518/hxkq.2020.03.002

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Chondrocyte cocultures with stromal vascular fraction of adipose tissue promote cartilage regeneration in vivo

Ba Kai1, Ni Duan2, Wang Xinbo1, Wei Xueqin1, Li Na1, Zheng Lian1()   

  1. 1.Dept. of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China
    2.Dept. of Emergency Medicine, Armed Police Corps Hospital of Henan, Zhengzhou 450000, China
  • Received:2019-05-15 Revised:2020-03-13 Online:2020-06-01 Published:2020-05-28
  • Contact: Lian Zheng E-mail:Zhenglian0726@163.com
  • Supported by:
    Supported by: The National Natural Science Foundation of China(81500826);The Youth Foundation of the First Affiliated Hospital of Zhengzhou University


Objective This study aimed to compare the cartilage regeneration of the stromal vascular fraction (SVF) cells and adipose-derived mesenchymal stem cells (ASCs) cocultured with chondrocytes seeded on the scaffolds. Methods The cellular morphologies and proliferation capabilities on the scaffolds were evaluated. The scaffolds with the coculture of ASCs/SVF and chondrocytes were implanted into the full thickness cartilage defective rabbit joints for 10 weeks. Results The cells seeded into the scaffolds showed good adhesion and proliferation. Implantation with SVF and chondrocytes revealed desirable in vitro healing outcomes. Conclusion The SVF cells were better than ASCs in terms of the formation of cartilage matrix in a coimplantation model. Without in vitro expansion, the SVF cells are good cell sources for cartilage repair.

Key words: chondrocytes, stromal vascular fraction, cocultures, cartilage regeneration

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