West China Journal of Stomatology ›› 2018, Vol. 36 ›› Issue (1): 23-28.doi: 10.7518/hxkq.2018.01.005

• Orginal Article • Previous Articles     Next Articles

Neurotrophin 3 promotes osteogenic differentiation of human dental follicle cells

Wei Lei1,2(), Yue Ma1,2, Aishu Ren1,3()   

  1. 1. Dept. of Orthodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China
    2. Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China
    3. Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China
  • Received:2017-06-10 Revised:2017-10-12 Online:2018-02-07 Published:2018-02-01
  • Supported by:
    Supported by: China Postdoctoral Science Foundation (2013M542248);Program for Innovation Team Building at Institutions of Higher Education in Chongqing in 2016 (CXTDG201602006);Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education [(2014)55].


Objective This study aims to investigate the effect of neurotrophin 3 (NT-3) on the osteogenic differentiation of human dental follicle cells (hDFCs). Methods hDFCs were isolated and cultured in vitro. Immunocytochemical staining was used to identify the origin of hDFCs. The effects of different NT-3 concentrations on hDFCs proliferation were detected by using CCK-8 assay. The alkaline phosphatase (ALP) activities and mRNA expression levels of bone morphogenetic protein-2 (BMP-2) and osteocalcin (OCN) were determined to investigate the effects of NT-3 on hDFCs osteogenesis. The difference in the number of mineralized nodules was detected using alizarin red staining. Results Vimentin and cytokeratin staining results showed that hDFCs originated from the mesenchymal cells. NT-3 exerted no evident effect on hDFCs proliferation. The ALP activity and the BMP-2 and OCN mRNA expression levels of hDFCs were significantly improved under treatment with different NT-3 concentrations (25, 50, and 100 ng·mL-1) compared with those in the control group. BMP-2 and OCN mRNA relative expression levels of hDFCs reached the highest when the NT-3 concentration was 100 ng·mL-1. The number of mine-ralized nodules reached the maximum when the hDFCs were treated with 50 and 100 ng·mL-1 NT-3. Conclusion Appropriate mass concentration of NT-3 can promote the osteogenic differentiation of hDFCs.

Key words: neurotrophin 3, human dental follicle cells, osteogenic differentiation

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