Comparative study with the effect of stromal cell derived factor-1 on osteogenic differentiation of human healthy and inflammatory periodontal ligament stem cells

doi:10.7518/hxkq.2019.05.003

Abstract

Abstract:

Objective This study aims to compare the osteogenic differentiation capability of stem cells derived from human inflammatory periodontal ligament tissues (iPDLSCs) with those of stem cells derived from healthy periodontal ligament tissues (hPDLSCs). Both types of tissues were induced by stromal cell derived factor (SDF-1) in vitro. Methods iPDLSCs and hPDLSCs were primarily cultured by tissue digestion method and purified by limited dilution cloning. The cells were passaged and identified by stem cell surface marker expression through flow cytometry. Then, we used thiazolyl blue tetrazolium bromide to detect and compare the proliferation capabilities of the iPDLSCs and hPDLSCs. Express of bone volumes were detected by alizarin red staining after SDF-1 was added to the cells. Using alkaline phosphatase, we evaluated the osteogenic differentiation capability of the cells induced by SDF-1. The expression levels of the osteogenesis-related genes of the cells induced by SDF-1 were determined by reverse transcription-polymerase chain reaction. Results After purification, both iPDLSCs and hPDLSCs expressed stem cell markers. hPDLCSs had a higher proliferation capability than iPDLSCs. Osteogenesis-related genes had higher expression levels in the cells induced by SDF-1 than in those without induction (P<0.05). SDF-1 at 50 and 200 ng·mL ^-1 concentration greatly affected the differentiation capabilities of iPDLSCs and hPDLSCs respectively. Conclusion iPDLSCs and hPDLSCs had osteogenic differentiation capability. The level of osteogenic differentiation in normal and inflamed periodontal ligament stem cells increases after SDF-1 induction.

Key words: stromal cell derived factor-1, inflammation, human periodontal ligament stem cells, osteogenic differentiation

CLC Number:

R780.2

Lin Yongsheng,Wang Fengzhi,Lei Xiaojing,He Jianmin. Comparative study with the effect of stromal cell derived factor-1 on osteogenic differentiation of human healthy and inflammatory periodontal ligament stem cells[J]. West China Journal of Stomatology, 2019, 37(5): 469-475.

Figures/Tables 6

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基因	编号	引物序列
Runx2	NM001015051	F：5’-TGCCAGCTGCATCCTATTT-3’
		R：5’-CCCTAAAGTCACTCGGTATGTG-3’
ColⅠ	BC054498	F：5’-AGAGTGGAGCAGTGGTTACTA-3’
		R：5’-GATACAGGTTTCGCCAGTAGAG-3’
OPN	AB469789	F：5’-CGAGGAGTTGAATGGTGCATA-3’
		R：5’-TCCAGCTGACTCGTTTCATAAC-3’
OCN	NM199173	F：5’-TCACACTCCTCGCCCTATT-3’
		R：5’-CCTCCTGCTTGGACACAAA-3’
BMP-2	NM001200	F：5’-GGGTGGAATGACTGGATTGT-3’
		R：5’-GAGTTCAGATGATCAGCCAGAG-3’
β-actin	NM001101	F：5’-GGGTGGAATGACTGGATTGT-3’
		R：5’-GACAGGATGCAGAAGGAGAT-3’