West China Journal of Stomatology ›› 2017, Vol. 35 ›› Issue (1): 51-56.doi: 10.7518/hxkq.2017.01.007

• Orginal Article • Previous Articles     Next Articles

Development and characterization of oriented scaffolds derived from cartilage extracellular matrix

Kun Li1,2(), Yanhong Zhao1(), Chen Xu1, Lianyong Wang3, Qiang Yang4, Hongfa Li1, Binhong Teng1   

  1. 1. Dept. of Orthodontics, Stomatological Hospital of Tianjin Medical University, Tianjin 300070, China
    2. Dept. of Orthodontics, Yantai Stomatological Hospital, Yantai 264000, China
    3. The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China
    4. Dept. of Spine Surgery, Tianjin Hospital, Tianjin 300211, China
  • Received:2016-06-20 Revised:2016-08-10 Online:2017-02-01 Published:2017-02-01
  • Supported by:
    The National Natural Science Foundation of China (31300798, 31470937, 81572154);The Key Project of Science and Technology Research of Tianjin Health Bureau (15KG125, 16KG114)


Objective This study aimed to prepare oriented scaffolds derived from a cartilage extracellular matrix (CECM) and to investigate their physicochemical property and compatibility with adipose-derived stem cells (ADSCs). Methods A fresh porcine articular cartilage was cut into pieces. Cartilage nanofibers with diameters of 50-500 nm were collected through homogenization and centrifugation. These nanofibers were then decellularized by using Triton X-100 to produce 6% CECM. The oriented scaffolds derived from the nanoscale CECM were fabricated through unidirectional solidification and lyophili-zation. Afterward, these scaffolds were crosslinked. The physical and chemical performances and cell compatibility of CECM-oriented scaffolds were evaluated. Results The cross-sections of the scaffolds contained homogeneous reticular porous struc-tures with nanofibers on the walls of the pores, and the longitudinal sections revealed vertical tubular structures. Hematoxylin-eosin staining revealed that the scaffolds were red without blue. Toluidine blue, safranin O, and Sirius red staining showed positive results. The porosity, water absorption rate, and vertical compressive elastic modulus of the scaffolds were 95.455%±0.910%, 95.889%±1.071%, and (40.208±5.097) kPa, respectively. Conclusion The components of the oriented scaffolds derived from CECM are similar to those of native cartilage with favorable biocompatibility. The porous structures and sizes of the scaffolds are suitable for the adhesion, prolife-ration, and infiltration of ADSCs. The oriented scaffolds derived from CECM are relatively optimal for cartilage tissue engineering.

Key words: cartilage, extracellular matrix, oriented scaffolds, tissue engineering, unidirectional solidification, lyophilization

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