Abstract:

Objective　To study the cell biocompatibility of porous biphasic calcium phosphate nanocompositein vitro.Meth- ods　Bone marrowmesenchymal cell(BMSCs) obtained from SD rat bone marrow werein vitroinduced and proliferated. After theirosteoblast phenotypes were verified, BMSCs were seeded onto prepared porous biphasic calcium phosphate nanocomposite (Experiment group) and common porous hydroxyapatite (Control group). The cell adhesion was evaluated by scanning electron microscope. Synthesis of alkaline phosphatase enzyme (ALP) and osteocalcin were detected and cell cycle was detected by flow cytometry.Results　BMSCs could fully attach to and extend on the material in experiment and control group, Moreover, experi- ment groupwere superiorto control group in adhesion, proliferative abilities and osteogenic activity.Conclusion　BMSCs can dif- ferentiate to osteoblast phenotype; the porous biphasic calcium phosphate nanocomposite as bone tissue engineering scaffold has good cell biocompatibility.

Key words: bone marrow stromal cells, porous biphasic calcium phosphate nanocomposite, tissue engineering