Study on Tumor Necrosis Factor-αand Interleukin 2 in Rat Skin Allograft

Abstract

Abstract:

Objective　To elucidate the histocompatibility of tissue engineered rat skin through studying the effect ofTNF- αand IL-2 in immunological rejection after rat skin allograft.Methods　Tissue engineered skin thatthe basic materialswere taken from neonatal SD rats was cultured in lab, grafted to adult Wistar rats. The expressions of TNF-αand IL-2 in grafted tissue were detected with immunohistochemical staining and Western blot.Results　The expressions of TNF-αand IL-2 were remarkable in skin allograft group, but low in tissue engineered skin group.Conclusion　The expressions of TNF-αand IL-2 and the immuno- logical rejection were closely related after skin allograft, but the tissue engineered skin has favorable histocompatibility and doesn′t arose obvious immunological rejections.

Key words: tumor necrosis factor-α, interleukin-2, tissue engineering, allograft

WANGJunlin,JINYan,LIUXiaoliang.. Study on Tumor Necrosis Factor-αand Interleukin 2 in Rat Skin Allograft[J]. West China Journal of Stomatology.

[1]	Tang Meng, Cui Zhan-qin, Wang Yangyang, Chen Zengguo, Li Wenjing, Zhang Cuiping. Effects of low-level laser on the expression of interleukin-6, tumor necrosis factor‑α, osteoprotegerin, and receptor activator of nuclear factor-κB ligand in human periodontal ligament cells [J]. West China Journal of Stomatology, 2023, 41(5): 521-532.
[2]	Dai Zhenning, Zheng Weihan, Li Shiyu. Receptor activator of nuclear factor-κB ligand and tumor necrosis factor-α promotes osteoclast differentiation through the exosomes of inflammatory periodontal ligament stem cells [J]. West China Journal of Stomatology, 2022, 40(4): 377-385.
[3]	Zhou Jian, Su Yingying, Wang Song-ling. Progress and perspectives on cell-free regenerative endodontic therapy [J]. West China Journal of Stomatology, 2022, 40(1): 1-6.
[4]	Lin Yunfeng. Research progress on the application of framework nucleic acid in bone regeneration [J]. West China Journal of Stomatology, 2021, 39(6): 624-632.
[5]	Jiang Xinquan. Advances in biomimetic modification of materials for oromaxillofacial bone regeneration and dental implant [J]. West China Journal of Stomatology, 2021, 39(2): 123-128.
[6]	Lai Jing, Bai Yuanliang, Bai Yin, Mei Jie, Zhang Zhiwei, Tang Wenjing, Huang Jiao. A Meta-analysis of the effect of non-surgical periodontal therapy on inflammatory factors in patients with chronic kidney disease and periodontitis [J]. West China Journal of Stomatology, 2020, 38(6): 672-680.
[7]	Wu Xiangnan, Ma Yuanyuan, Hao Zhichao, Wang Hang. Research progress on the biological regulatory function of lysophosphatidic acid in bone tissue cells [J]. West China Journal of Stomatology, 2020, 38(3): 324-329.
[8]	Zhao Dan,Li Yueheng,Yang Zhengyan,Cai Ting,Wu Xiaoyan,Xia Yu,Zhou Zhi. Effect of the local application of stem cells on repairing facial nerve defects: a systematic review [J]. West China Journal of Stomatology, 2020, 38(1): 59-68.
[9]	Yunjie Li,Binhong Teng,Yanhong Zhao,Qiang Yang,Lianyong Wang,Ying Huang. Preparation and evaluation of carboxymethyl chitosan/sodium alginate hydrogel for cartilage tissue engineering [J]. West China Journal of Stomatology, 2019, 37(3): 253-259.
[10]	Yubin Cao,Chang Liu,Weilin Pan,Yuan Tu,Chunjie Li,Chengge Hua. Research progress on the modification of guided bone regeneration membranes [J]. West China Journal of Stomatology, 2019, 37(3): 325-329.
[11]	Yunjie Li,Yanhong Zhao,Qiang Yang. Development of cartilage extracellular matrix in cartilage tissue engineering [J]. West China Journal of Stomatology, 2019, 37(2): 220-223.
[12]	Tian Zhu,Weihua. Guo. Dentin matrix in tissue regeneration: a progress report [J]. West China Journal of Stomatology, 2019, 37(1): 92-96.
[13]	Yun Yang,Shanshan Chen,Chunmei Xu,Yafei Wu,Lei Zhao. Expression of triggering receptors expressed by myeloid cells-1 in macrophages stimulated by Porphyromonas gingivalis-lipopolysaccharide [J]. West China Journal of Stomatology, 2018, 36(5): 475-481.
[14]	Xinxin Ding, Yanmin Zhou, Xing-chen Xiang, Lin Meng, Qin Qin, Shan Ye. Research progress on chitosan composite scaffolds in bone tissue engineering [J]. West China Journal of Stomatology, 2018, 36(4): 441-446.
[15]	Binhong Teng, Yanhong Zhao, Lianyong Wang, Qiang Yang, Hongfa Li, Yunjie Li. Preparation and characterization of oriented scaffolds derived from cartilage extracellular matrix and silk fibroin [J]. West China Journal of Stomatology, 2018, 36(1): 17-22.

Study on Tumor Necrosis Factor-αand Interleukin 2 in Rat Skin Allograft

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments