原子力显微镜分析激光消融对牙本质表面微纳米结构的影响

doi:10.7518/hxkq.2022.02.005

华西口腔医学杂志 ›› 2022, Vol. 40 ›› Issue (2): 155-161.doi: 10.7518/hxkq.2022.02.005

原子力显微镜分析激光消融对牙本质表面微纳米结构的影响

林琪(), 林昱, 谢云德, 马忠雄()

福建医科大学附属口腔医院口腔黏膜科，福建省口腔疾病重点实验室，福州 350002

收稿日期:2021-04-28 修回日期:2022-01-10 出版日期:2022-04-01 发布日期:2022-04-01
通讯作者: 马忠雄 E-mail:Linqifj@163.com;372590301@qq.com
作者简介:林琪，主治医师，硕士，E-mail：Linqifj@163.com
基金资助:
福建省教育厅青年科研项目(JAT170256)

Atomic force microscopy observation of the effect of laser ablation on the nanostructures of the dentin surface

Lin Qi(), Lin Yu, Xie Yunde, Ma Zhongxiong.()

Dept. of Mucous, the Affiliated Stomatological Hospital, Fujian Medical University; Fujian Key Laboratory of Oral Diseases, Fuzhou 350002, China

Received:2021-04-28 Revised:2022-01-10 Online:2022-04-01 Published:2022-04-01
Contact: Ma Zhongxiong. E-mail:Linqifj@163.com;372590301@qq.com
Supported by:
Youth Scientific Research Project of Education Department of Fujian Province(JAT170256);Correspondence: Ma Zhongxiong, E-mail: 372590301@qq.com

摘要/Abstract

摘要： 目的

运用原子力显微镜（AFM）分析不同能量密度激光辐照对人牙本质表面有机质及微纳米晶体结构的影响。

方法

收集新鲜拔除的无龋、无损伤、无脱矿及无发育不良的人第三磨牙，制作厚度为1.0 mm的牙本质切片样本，样本采用随机区组设计，用6.18、8.04、9.89、11.1 J·cm^-2能量密度的激光面扫描人牙本质，运用AFM的相位成像技术及扫描电镜的能谱仪分析该激光照射前后的牙本质表面有机物成分及微纳米晶体结构改变。

结果

经AFM相位成像技术分析，随着激光照射能量密度的增加，牙本质表面有机质所占的比例减少，无机质所占的比例增加；但牙本质表面无熔融现象，牙本质羟磷灰石纳米晶体颗粒相互团聚，但仍可见颗粒间存在清晰的界限及颗粒形态，未见完全融合、增大的颗粒。能谱仪分析表明，牙本质表面元素主要为Ca、P、O、C、Na、Mg、Cl等，LSD检验结果显示，激光照射后C、O、Na元素及C/Ca比值下降，Ca、P元素及Ca/P比值升高（P<0.05）。

结论

6.18、8.04、9.89、11.1 J·cm^-2能量密度的激光辐照人牙本质，会造成牙本质表面有机质的减少，但不会明显改变牙本质微纳米晶体结构。

关键词: 原子力显微镜, 相位成像, 牙本质, 激光消融, 能谱仪

Abstract: Objective

An atomic force microscope (AFM) was used to analyze the changes in organic matter in human dentin and the effects of laser irradiation with different energy densities on the surface micro-nanostructures of human dentin.

Methods

Extracted human third molars were used in this study. Human dentin were scanned with an Er, Cr:YSGG laser (6.18, 8.04, 9.89, 11.1 J·cm^-2). The 12 samples were randomly allocated. AFM phase imaging technology and energy-dispersive spectroscopy (EDS) were applied to detect the proportional changes in the organic components on the dentine surface before and after Er,Cr:YSGG laser irradiation. AFM was capable of observing the morphological changes of the dentine surface and hydroxyapatite crystals before and after Er, Cr:YSGG laser irradiation.

Results

After laser irradiation, the proportion of collagen with low contrast in the phase images remarkably reduced. Accordingly, the proportion of the inorganic phase significantly increased. EDS results showed that dentine was composed of Ca, P, O, and C and some trace elements of Na, Mg and Cl. After Er, Cr:YSGG laser irradiation, the C, O, and Na contents and C/Ca ratio declined significantly (P<0.05), whereas the Ca and P contents and the Ca/P ratio obviously increased (P<0.05).

Conclusion

Irradiating human dentine with an Er, Cr:YSGG laser at varying energy densities from 6.18 to 11.1 J·cm^-2 did not significantly influence the inorganic phase structure of the surface dentine layer. However, thermal ablation occurred in the organic component.

Key words: atomic force microscope, phase ima-ging, dentin, laser ablation, energy-dispersive spectroscopy

中图分类号:

R 781

林琪, 林昱, 谢云德, 马忠雄. 原子力显微镜分析激光消融对牙本质表面微纳米结构的影响[J]. 华西口腔医学杂志, 2022, 40(2): 155-161.

Lin Qi, Lin Yu, Xie Yunde, Ma Zhongxiong.. Atomic force microscopy observation of the effect of laser ablation on the nanostructures of the dentin surface[J]. West China Journal of Stomatology, 2022, 40(2): 155-161.

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参考文献 16

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成分	区组	激光强度/（J·cm^-2）
成分	区组	0	6.18	8.04	9.89	11.1
C	1－12	9.22±2.525^a	5.53±2.059^b	5.62±2.345^b	5.71±2.539^b	5.37±2.095^b
O	1－12	47.93±5.455^a	41.92±5.479^b	41.57±5.208^b	40.41±3.593^b	40.39±4.160^b
Ca	1－12	20.22±4.102^a	27.65±4.623^b	27.94±5.256^b	28.91±3.637^b	29.44±4.422^b
P	1－12	15.63±1.379^a	18.36±1.248^b	18.30±1.317^b	18.31±1.083^b	18.44±0.819^b
Na	1－12	2.03±0.716^a	1.72±0.837^b	1.69±0.716^b	1.64±0.580^b	1.55±0.638^b
Mg	1－12	1.11±0.252^a	1.19±0.247^a	1.21±0.260^a	1.23±0.320^a	1.16±0.297^a
C/Ca	1－12	0.50±0.230^a	0.22±0.146^b	0.23±0.171^b	0.21±0.136^b	0.19±0.107^b
Ca/P	1－12	1.28±0.159^a	1.50±0.184^b	1.52±0.205^b	1.57±0.142^b	1.59±0.210^b

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原子力显微镜分析激光消融对牙本质表面微纳米结构的影响

Atomic force microscopy observation of the effect of laser ablation on the nanostructures of the dentin surface

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