脱矿牙本质基质和脱细胞牙本质基质成骨效果的对比研究

doi:10.7518/hxkq.2023.2023161

华西口腔医学杂志 ›› 2024, Vol. 42 ›› Issue (1): 28-36.doi: 10.7518/hxkq.2023.2023161

脱矿牙本质基质和脱细胞牙本质基质成骨效果的对比研究

达尔亚·俄尼木拜¹(), 张迪¹, 古丽努尔·阿吾提¹^,²()

^1.新疆医科大学第一附属医院/附属口腔医（学）院牙周病科，乌鲁木齐 830011
^2.新疆维吾尔自治区口腔医学研究所，乌鲁木齐 830011

收稿日期:2023-05-24 修回日期:2023-07-27 出版日期:2024-02-01 发布日期:2024-01-12
通讯作者: 古丽努尔·阿吾提 E-mail:675151369@qq.com;guawuti@sina.com
作者简介:达尔亚·俄尼木拜，硕士，E-mail：675151369@qq.com
基金资助:
中华口腔医学会西部口腔医学临床科研基金(CSA-W-2020-11)

Comparison of the osteogenic effects of demineralized dentin matrix and acellular dentin matrix

Enimubai Daerya¹(), Zhang Di¹, Awuti Gulinuer¹^,²()

^1.Dept. of Periodontology, The First Affiliated Hospital of Xinjiang Medical University/Affiliated College of Stomatology, Urumqi 830011, China
^2.Stomatology Disease Institute of Xinjiang Uyghur Autonomous Region, Urumqi 830011, China

Received:2023-05-24 Revised:2023-07-27 Online:2024-02-01 Published:2024-01-12
Contact: Awuti Gulinuer E-mail:675151369@qq.com;guawuti@sina.com
Supported by:
Western Clinical Research Foundation of Stomatology of Chinese Academy of Stomatology(CSA-W2020-11);Correspondence: Gulinuer Awuti, E-mail: guawuti@sina.com

摘要/Abstract

摘要：

目的比较骨缺损区植入脱矿牙本质基质和脱细胞牙本质基质的成骨效果。方法制备脱矿牙本质基质和脱细胞牙本质基质。将24只SPF级SD雄性大鼠随机分为脱矿组（A组）、脱细胞组（B组）、Bio-Oss骨粉组（C组）、空白组（D组），每组6只大鼠，在麻醉条件下制备双侧股骨骨缺损。A、B、C组大鼠分别在骨缺损区植入脱矿牙本质基质、脱细胞牙本质基质、Bio-Oss骨粉，D组大鼠不植入任何材料。术后4周和8周，每组各随机处死3只大鼠。大体观察骨缺损区愈合情况，血清学检测成骨指标骨形态发生蛋白（BMP）-2及碱性磷酸酶（ALP）浓度，影像学观察骨缺损区高密度灰色区（代表骨愈合）分布情况，组织形态学观察新骨形成情况，计算新骨形成率。结果术后4周和8周，大体观察见A组成骨能力较其他组活跃，血清学检测A组BMP-2及ALP浓度均高于其他组，差异有统计学意义（P<0.05）。8周时，影像学观察可见A组骨缺损区高密度灰色区分布均匀，组织形态学观察见A组排列规则的骨基质。A组4、8周时的新骨形成率分别为28.51%±0.55%、32.57%±2.28%，均高于其他组，差异有统计学意义（P<0.05）。

关键词: 脱矿牙本质基质, 脱细胞牙本质基质, 骨修复, 骨移植材料

Abstract:

Objective This study aims to compare the osteogenic effects of implanting demineralized dentin matrix and acellular dentin matrix in bone defect areas. Methods Demineralized dentin matrix and acellular dentin matrix were prepared. Twenty-four male SPF-grade SD rats were randomly divided into four groups: demineralized group (group A), acelluar group (group B), Bio-Oss bone powder group (group C), and blank control group (group D), with six rats in each group. All rats were subjected to general anesthesia to prepare bilateral femoral bone defects. Rats in groups A, B, and C were implanted with demineralized dentin matrix, acellular dentin matrix, and Bio-Oss bone powder at the bone defect area, respectively, while rats in group D were not implanted with any material. At 4 and 8 weeks after surgery, three rats were randomly executed in each group. The healing of the bone defect area was analyzed through gross observation. The concentrations of osteogenic indicators bone morphogenetic protein-2 (BMP-2) and alkaline phosphatase (ALP) were detected by serology. The distribution of high-density gray area (representing bone healing) in the bone defect area was observed by X-ray examination, and the formation of new bone was observed by histomorphology. The rate of new bone formation was calculated. Results At 4 and 8 weeks, the bone formation ability of group A was more active than that of the other groups; the concentrations of BMP-2 and ALP in group A were higher than those in the other groups, and the differences were statistically significant (P<0.05). At 8 weeks, the imaging observation showed that the high-density gray area in the bone defect of group A was evenly distributed. The histomorphological observation showed the regular arrangement of bone matrix in group A. The rate of new bone formation in group A was 28.51%±0.55% at 4 weeks and 32.57%±2.28% at 8 weeks, both of which were significantly higher than those in the other groups (P<0.05). Conclusion Demineralized dentin matrix has better osteogenic potential than acellular dentin matrix.

Key words: demineralized dentin matrix, acellular dentin matrix, bone repair, bone graft material

中图分类号:

达尔亚·俄尼木拜, 张迪, 古丽努尔·阿吾提. 脱矿牙本质基质和脱细胞牙本质基质成骨效果的对比研究[J]. 华西口腔医学杂志, 2024, 42(1): 28-36.

Enimubai Daerya, Zhang Di, Awuti Gulinuer. Comparison of the osteogenic effects of demineralized dentin matrix and acellular dentin matrix[J]. West China Journal of Stomatology, 2024, 42(1): 28-36.

图/表 5

图 1

表 1

表 2

图 2

图 3

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组别	4周	8周	P值
P值	0.004	0.047
A组	3.923 3±0.305 5	3.696 7±0.680 7	0.596
B组	3.670 0±0.624 5*	3.113 3±0.180 3^#	0.220
C组	3.680 0±0.108 1*	3.160 0±0.322 3^#	0.248
D组	3.573 3±0.971 3*	3.040 0±0.341 7^#	0.198

组别	4周	8周	P值
P值	0.002	0.015
A组	4.156 7±0.299 3	3.616 7±0.305 5	0.287
B组	3.710 0±0.125 3*	3.473 3±0.404 1^#	0.619
C组	3.446 7±0.757 0*	3.420 0±0.104 4^#	0.521
D组	3.426 7±0.642 9*	3.376 7±0.802 1^#	0.188

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脱矿牙本质基质和脱细胞牙本质基质成骨效果的对比研究

Comparison of the osteogenic effects of demineralized dentin matrix and acellular dentin matrix

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