Abstract:

Objective　To investigate the chondrogenisis by alginate gelatin and rats′bone marrowstromal cells(BMSCs) chon- drogenicly inducedin vitro.Methods　Thirty-two male adult SD ratswere assigned randomly to experimental and control groups. In experimental group, bone marrowwas obtained from the right tibias of all the rats. After expanding and culturing 3 passages, induced BMSCs by chondrogenic culture medium for 10 days. Suspended induced cells in alginate gelatin, and injected the com- plex into the hypodermic tissue of the backs of rats autogenously. In control group only alginate gelatins were injected. The grafts were taken out for examinations 4 and 8 weeks after the operations.Results　Considerable cartilage appeared in experimental group 8 weeks after operations. RegularHE staining and alcian blue staining showed a great deal of cartilage holding chondrocyte masses surrounded by abundant matrix. Alginate gelatin decompounded obviously, and the rest distributed among newly formed cartilage. No cartilage appeared in control group all through.Conclusion　BMSCs and alginate gelatin have a beautiful future in cartilage tissue engineering.

Key words: mesenchymal stromal cells(MSCs), alginate gelatin, cartilage, tissue engineering