Differences in near-infrared fluorescence imaging and histological analysis of cheek mucosa in golden hamsters with different pathological states

doi:10.7518/hxkq.2024.2024150

Abstract

Abstract:

Objective To explore and analyze the feasibility of using indocyanine green (ICG) near-infrared fluorescence (NIF) imaging technology for the early diagnosis of oral potential malignant disorders and oral squamous cell carcinoma. Methods 7,12-Dimethylbenz[a]anthracene in acetone solution was used to induce various pathological models of buccal mucosal lesions (mild/moderate dysplasia, severe dysplasia, squamous cell carcinoma) in golden hamster. ICG-NIF was conducted for the quantitative analysis of the fluorescence signal of lesion tissue, and evaluation of the diagnostic and discriminative capabilities of the ICG-NIF technology for mucosal lesions in various pathological states. Immunohistochemical staining was perform to examine the microvessel density (MVD) and microlymphatic vessel density (MLVD) of mucosa in various pathological states and explore the histological reasons underlying the differences in fluorescence signals. Results The results of ICG-NIF fluorescence quantitative analysis reveal the higher fluorescence intensity of mucosal lesions in the experimental group compared with that of the normal mucosa on the control side, with statistical differences (P<0.05). Moreover, the more severe the malignancy of mucosal lesions in the experimental group, the higher the fluorescence intensity. According to histopathological analysis, the malignant progression of mucosal lesions in golden hamsters was accompanied with an increase in MVD (P<0.05) and a decrease in MLVD (P<0.05). Conclusion The abnormal proliferation of mucosal lesions in golden hamsters exhibits a difference in ICG-NIF fluorescence signal compared with normal mucosal tissue. Fluorescence quantitative analysis methods can provide assistance in differentiation and show potential for clinical applications.

Key words: indocyanine green, near-infrared fluorescence imaging, oral potentially malignant disorders, oral squamous cell carcinoma

CLC Number:

R780.2

Xie Diya, Shan Danni, Zhang Lei, Chen Sheng, Na Yingyu, Wang Zhiyong. Differences in near-infrared fluorescence imaging and histological analysis of cheek mucosa in golden hamsters with different pathological states[J]. West China Journal of Stomatology, 2024, 42(6): 716-722.

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组别	MFI	SBR	MVD	MLVD
对照组	65±9.16	-	14.4±2.97	7.8±2.59
轻/中度异常增生组	123±12.26	1.89±0.32	26.4 ±5.32	6.4±1.64
重度异常增生组	214±21.12	3.27±0.46	73.2±11.14	5.6±1.32
OSCC组	325±47.46	4.97±0.87	101.2±15.30	4.2±1.14

技术	检测深度/mm	优势	不足
自体荧光成像	-	操作简便，阴性预测值较高	特异性较低
窄带成像技术	0.17~0.20	显示上皮内血管，提高组织表面结构的对比度	穿透深度不足，角化物影响血管可见性
反射式共聚焦显微镜	0.2~03	高分辨率显示亚细胞结构	穿透深度不足，角化物影响观察
光学相干断层扫描技术	1.5~2	提供口腔组织微观结构和微血管的二维或三维成像	机械压力会影响，软组织的光学特征
ICG-NIF成像	5~10	足够的穿透深度，可定量分析	需注射荧光显影剂

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