Expression and mechanism of long non-coding RNA HCG22 in oral squamous cell carcinoma

doi:10.7518/hxkq.2021.06.006

Abstract

Abstract: Objective

To investigate the expression and mechanism of the long non-coding RNA (lncRNA) HCG22 in oral squamous cell carcinoma (OSCC).

Methods

HCG22 levels were detected in the OSCC and adjacent tissues, OSCC cells, and normal oral keratinocytes. HCG22 expression in SCC-25 and HSC-3 cells was upregulated by transfection of the overexpressing plasmi dvector. Methyl thiazolyl tetrazolium (MTT) assay, flow cytometry, and Transwell assay were employed to detect changes in cell proliferation, apoptosis, migration, and invasion ability, while Western blotting was used to detect the expression of epithelial-mesenchymal transformation-related proteins. The expression level of miR-650 in the cells was detected by real-time quantitative polymerase chain reaction (RT-qPCR), and dual-luciferase reporter gene assay was applied to assess the targeting relationship between HCG22 and miR-650.

Results

Compared with that in adjacent tissues, the expression of HCG22 significantly decreased in OSCC tissues (P<0.05). Moreover, the prognostic survival of patients in the low-HCG22 expression group was significantly lower than that in the high-expression group (P<0.05). Compared with that in HOK cells, the expression of HCG22 was significantly lower in SCC-25, HN13, HSC-3, and CAL-27 cells (P<0.05). Upregulation of HCG22 expression could inhibit the proliferation, migration, invasion, and apoptosis of SCC-25 and HSC-3 cells, upregulatethe expression of E-cadherin, and downregulate the expression of N-cadherin and vimentin (P<0.05). miR-650 mimics could reduce the luciferase activity of HCG22 wild-type plasmid cells (P<0.05), and the expression of miR-650 in SCC-25 and HSC-3 cells decreased after upregulation of HCG22 expression (P<0.05).

Conclusion

HCG22 is expressed at low levels in OSCC. Upregulation of the expression of this lncRNA can inhibit the proliferation, migration, invasion, and epithelial-mesenchymal transition of OSCC cells. The mechanism of action of HCG22 may be related to its targeted regulation of miR-650.

Key words: oral squamous cell carcinoma, long non-coding RNA HCG22, cell migration, cell invasion, microRNA-650

CLC Number:

Q 786

Gao Yongqiang, Shi Pengwei, Shi Wenkai, Liu Yiming. Expression and mechanism of long non-coding RNA HCG22 in oral squamous cell carcinoma[J]. West China Journal of Stomatology, 2021, 39(6): 658-666.

Figures/Tables 8

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资料		n	HCG22［n（%）］		χ²值	P值
资料		n	低表达组	高表达组	χ²值	P值
性别	男	36	20（55.56）	16（44.44）	0.846	0.358
	女	32	20（62.50）	12（37.50）
年龄/岁	>60	39	22（56.41）	17（43.59）	0.744	0.388
	≤60	29	18（62.07）	11（37.93）
肿瘤大小/cm	≥3	41	21（51.22）	20（48.78）	7.553	0.006
	<3	27	19（70.37）	8（29.63）
肿瘤部位	舌部	38	22（57.89）	16（42.11）	0.111	0.946
	颊部	20	12（60.00）	8（40.00）
	其他	10	6（60.00）	4（40.00）
TNM 分期	Ⅰ~Ⅱ期	40	26（65.00）	14（35.00）	4.604	0.032
	Ⅲ~Ⅳ期	28	14（50.00）	14（50.00）
分化程度	低分化	42	27（64.29）	15（35.71）	3.998	0.046
	中、高分化	26	13（50.00）	13（50.00）
淋巴结转移	无	47	30（63.83）	17（36.17）	5.195	0.023
	有	21	10（47.62）	11（52.38）

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