Effect of microRNA-663b on migration, invasion and epithelial‑mesenchymal transition of oral squamous cell carcinoma cells

doi:10.7518/hxkq.2022.04.003

Abstract

Abstract:

Objective To explore the effect of microRNA-663b (miR-6636) on migration, invasion and epithelial-mesenchymal transition (EMT) of oral squamous cell carcinoma cells (OSCC). Methods Use R Studio of gene expression omnibus (GEO) database to analyze expressions of miR-663b in the OSCC and adjacent normal tissues. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of miR-663b in tissues and cells. The transfection efficiency of HN30 cells with miR-663b knockout plasmid was detected. Transwell assay was used to detect the effect of the migration and invasion ability. Bioinformatics method was used to predict the targeted mRNA that may bind to miR-663b and double luciferase assay was used to verify the binding. Western blot assay was used to detect the expression of EMT-related markers. Results The expression of miR-663b was up-regulated in OSCC tissues and higher in HN30, CAL27 and SCC-9 cells than in HOEC cells (P<0.05). Knockout of miR-663b could inhibit migration and invasion of HN30 cells (P<0.05) and inhibit the occurrence of EMT. Bioinformatics prediction software predicts that SH3BP2 was the target gene of miR-663b, and patients with low SH3BP2 expression had a poor prognosis (P<0.05). MiR-663b could bind to SHBP2 (P<0.05). The expression of SH3BP2 was increased and the occurrence of EMT was inhibited in HN30 cells with miR-663b knocked out. Conclusion Knockout of miR-663b can inhibit the migration, invasion and EMT of OSCC by targeting SH3BP2.

Key words: miR-663b, SH3BP2, oral squamous cell carcinoma, migration, invasion, epithelial-mesenchymal transition

CLC Number:

R739.8

Cong Biqiao, Liu Xiaoping, Chen Jiawen, Li Hongli, Fan Xin. Effect of microRNA-663b on migration, invasion and epithelial‑mesenchymal transition of oral squamous cell carcinoma cells[J]. West China Journal of Stomatology, 2022, 40(4): 386-393.

Figures/Tables 6

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References 20

1	Yang Z, Liang X, Fu Y, et al. Identification of AUNIP as a candidate diagnostic and prognostic biomarker for oral squamous cell carcinoma[J]. EBioMedicine, 2019, 47: 44-57.
2	Yen CJ, Tsou HH, Hsieh CY, et al. Sequential therapy of neoadjuvant biochemotherapy with cetuximab, paclitaxel, and cisplatin followed by cetuximab-based concurrent bioradiotherapy in high-risk locally advanced oral squamous cell carcinoma: final analysis of a phase 2 clinical trial[J]. Head Neck, 2019, 41(6): 1703-1712.
3	Guo J, Zhou S, Huang P, et al. NNK-mediated upregulation of DEPDC1 stimulates the progression of oral squamous cell carcinoma by inhibiting CYP27B1 expression[J]. Am J Cancer Res, 2020, 10(6): 1745-1760.
4	Sung H, Ferlay J, Siegel RL, et al. Global Cancer Statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2021,71(3): 209-249.
5	Fadaka AO, Pretorius A, Klein A. Biomarkers for stratification in colorectal cancer: microRNAs[J]. Cancer Control, 2019, 26(1): 1073274819862784.
6	O’Connell RM, Rao DS, Chaudhuri AA, et al. Physiological and pathological roles for microRNAs in the immune system[J]. Nat Rev Immunol, 2010, 10(2): 111-122.
7	Treiber T, Treiber N, Meister G. Regulation of microRNA biogenesis and its crosstalk with other cellular pathways[J]. Nat Rev Mol Cell Biol, 2019, 20(1): 5-20.
8	Wang S, Claret FX, Wu W. MicroRNAs as therapeutic targets in nasopharyngeal carcinoma[J]. Front Oncol, 2019, 9: 756.
9	Wang YL, Shen Y, Xu JP, et al. Pterostilbene suppresses human endometrial cancer cells in vitro by down-regula-ting miR-663b[J]. Acta Pharmacol Sin, 2017, 38(10): 1394-1400.
10	Wang M, Jia M, Yuan K. MicroRNA-663b promotes cell proliferation and epithelial mesenchymal transition by directly targeting SMAD7 in nasopharyngeal carcinoma[J]. Exp Ther Med, 2018, 16(4): 3129-3134.
11	Luo Y, Li W, Ling Z, et al. ASCT2 overexpression is associated with poor survival of OSCC patients and ASCT2 knockdown inhibited growth of glutamine-addicted OSCC cells[J]. Cancer Med, 2020, 9(10): 3489-3499.
12	Shu Y, Ye W, Gu YL, et al. Blockade of miR-663b inhibits cell proliferation and induces apoptosis in osteosarcoma via regulating TP73 expression[J]. Bratisl Lek Listy, 2018, 119(1): 41-46.
13	Du M, Shi D, Yuan L, et al. Circulating miR-497 and miR-663b in plasma are potential novel biomarkers for bladder cancer[J]. Sci Rep, 2015, 5: 10437.
14	Zhang Y, Weinberg RA. Epithelial-to-mesenchymal transition in cancer: complexity and opportunities[J]. Front Med, 2018, 12(4): 361-373.
15	Guarino M, Rubino B, Ballabio G. The role of epithelial-mesenchymal transition in cancer pathology[J]. Pathology, 2007, 39(3): 305-318.
16	Wang Z, Divanyan A, Jourd’heuil FL, et al. Vimentin expression is required for the development of EMT-related renal fibrosis following unilateral ureteral obstruction in mice[J]. Am J Physiol Renal Physiol, 2018, 315(4): F769-F780.
17	Mukai T, Gallant R, Ishida S, et al. Loss of SH3 domain-binding protein 2 function suppresses bone destruction in tumor necrosis factor-driven and collagen-induced arthritis in mice[J]. Arthritis Rheumatol, 2015, 67(3): 656-667.
18	Kawahara K, Mukai T, Iseki M, et al. SH3BP2 Deficiency Ameliorates Murine Systemic Lupus Erythematosus[J]. Int J Mol Sci, 2021,22(8): 4169.
19	Lin L, Chamberlain L, Pak ML, et al. A large-scale RNAi-based mouse tumorigenesis screen identifies new lung cancer tumor suppressors that repress FGFR signaling[J]. Cancer Discov, 2014, 4(10): 1168-1181.
20	Kittaka M, Yoshimoto T, Schlosser C, et al. Alveolar bone protection by targeting the SH3BP2-SYK axis in osteoclasts[J]. J Bone Miner Res, 2020, 35(2): 382-395.

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