West China Journal of Stomatology ›› 2024, Vol. 42 ›› Issue (1): 46-55.doi: 10.7518/hxkq.2024.2023237

• Basic Research • Previous Articles     Next Articles

miR-362-3p inhibited the invasion and metastasis of oral squamous cell carcinoma cells by targeting the regulation of pituitary tumor-transforming gene 1

Ding Xiao1,2(), Chen Jiawen2, Qu Pengyu1, Sun Chenyu1, Li Hongli3, Hu Wenting1, Fan Xin1()   

  1. 1.Dept. of Stomatology, Affiliated Hospital of Weifang Medical University, Weifang 261000, China
    2.School of Stomatology, Weifang Medical University, Weifang 261053, China
    3.Medical Research Center, Weifang Medical University, Weifang 261053, China
  • Received:2023-07-26 Revised:2023-12-12 Online:2024-02-01 Published:2024-01-12
  • Contact: Fan Xin E-mail:dx6666yy@126.com;fanxinsunny@aliyun.com
  • Supported by:
    Natural Science Foundation Project of Shandong Province(ZR202110190030);Fundamental Research Program Funding of Affiliated Hospital of Weifang Medical University(2021wffyzzjj06);Weifang Science and Technology Development Plan Project(2022YX029);Correspondence: Fan Xin, E-mail: fanxinsunny@aliyun.com

Abstract:

Objective This study aimed to explore the effect of pituitary tumor-transforming gene 1 (PTT-G1) on the invasion and proliferation of oral squamous cell carcinoma (OSCC) cell lines under the action of miR-362-3p. Methods The bioinformatics online database was used to query the expression of PTTG1 in head and neck squamous cell carcinoma (HNSCC). The expression of PTTG1 in the Cal-27, HN-30, and HOK cell lines was detected by Western blot. A wound-healing assay was used to determine the effect of PTTG1 on the migration ability of the OSCC cells. The Transwell assay was used to examine the changes in cell-invasion ability. 5-ethynyl-2'-deoxyuridine (EdU) cell-proliferation assay was used to detect changes in cell-proliferation ability. Bioinformatics approach predicted the upstream miRNA of PTTG1. The targeting relationship between miR-362-3p and PTTG1 was examined by the dual luciferase assay, and quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the expression of miRNA in OSCC tissues. Results The ENCORI database showed that PTTG1 expression was up-regulated in OSCC tissues. Western blot confirmed that PTTG1 expression was up-regulated in Cal-27 and HN-30 cells than HOK cells. PTTG1 knockout can inhibit the migration, invasion, and proliferation of Cal-27 and HN-30 cells (P<0.05). Bioinformatics prediction websites predicted that the upstream miRNA of PTTG1 was miR-362-3p, and PTTG1 can bind to miR-362-3p. Results of qRT-PCR showed that miR-362-3p expression was downregulated in OSCC tissues compared with normal tissue (P<0.05). Transwell and EdU experiments confirmed that miR-362-3p knockdown can promote the invasion and proliferation of Cal-27 and HN-30 after PTTG1 knockdown. Conclusion miR-362-3p can inhibit the invasion and proliferation of Cal-27 and HN-30 cells by targeting PTTG1.

Key words: oral squamous cell carcinoma, pituitary tumor-transforming gene 1, microRNA, invasion, proliferation

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