Effect of SOX2 gene on the biological characteristics of salivary adenoid cystic carcinoma cells

doi:10.7518/hxkq.2022.03.015

Abstract

Abstract: Objective

This study aimed to investigate the effect of transfecting SOX2-shRNA vector lentivirus to SACC cell lines on the biological behavior of salivary adenoid cystic carcinoma (SACC)-LM and SACC-83.

Methods

Three types of SOX2-shRNA lentiviral vectors (817, 818, and 819) were constructed and transfected successfully. The shRNA with the best inhibitory effect was screened out and transfected into SACC cells. Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and Western blot were used to detect the expressions of Survivin, E-cadherin, and N-cadherin of SACC-LM and SACC-83. CCK-8 and flow cytometry were used to detect SACC-LM and SACC-83 proliferation and apoptosis. Cell scratch test and Transwell method were used to detect the migration and invasion capabilities of SACC-LM and SACC-83.

Results

SOX2-shRNA-819 had the best interference effect among the three lentiviruses. After transfecting SOX2-shRNA-819 into SACC-LM and SACC-83, the expressions of SOX2, Survivin, and N-cadherin were significantly reduced, and that of E-cadherin was significantly increased (P<0.05). The cell proliferation ability decreased, and the number of apoptotic cells increased (P<0.05). The cell migration and invasion ability decreased (P<0.05).

Conclusion

shRNA interference technology reduced SOX2 expression while downregulating Survivin expression. These two expressions may be related. Low SOX2 expression inhibits the proliferation, migration, and invasion of SACC cells and promotes the apoptosis of SACC cells. SOX2 may be involved in the epithelial⁃mesenchymal transition process. This study provided a relevant theoretical basis for targeting SOX2 gene therapy in SACC.

Key words: salivary adenoid cystic carcinoma, SOX2, RNA interference, biological behavior, proliferation, migration, invasion, apoptosis, lentivirus

CLC Number:

R 739.8

Wei Zhuo, Zhou Hao, Fei Wei, Nie Xiong, Li Mingfang. Effect of SOX2 gene on the biological characteristics of salivary adenoid cystic carcinoma cells[J]. West China Journal of Stomatology, 2022, 40(3): 341-349.

Figures/Tables 12

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慢病毒载体	靶点序列
SOX2-shRNA-817	5’-CGCTCATGAAGAAGGATAAG-3’
SOX2-shRNA-818	5’-TGGACAGTTACGCGCACATG-3’
SOX2-shRNA-819	5’-CAGCTCGCAGACCTACATGA-3’
Negative-shRNA-376	5’-CGAGACACGAACGGAGAAGA-3’

组别	SOX2	E-cadherin	N-cadherin	Survivin
Mock组	1.110±0.105	0.646±0.049	1.102±0.035	1.170±0.057
pUc组	1.023±0.120	0.653±0.043	1.058±0.029	1.085±0.064
pU组	0.507±0.012^ab	0.792±0.050^ab	0.513±0.014^ab	0.631±0.096^ab

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