Impacts of periodontitis on visceral organ weight and weight percentage in obese mice

doi:10.7518/hxkq.2018.05.010

Abstract

Abstract:

Objective This study aimed to explore the impacts of periodontitis on the visceral weight and weight percen-tage of obese animal models. Methods A total of 64 C57BL/6J mice were divided into the following diet groups: high-fat diet (HFD) group (n=36), which was fed with high-fat diet to induce obesity, and low-fat diet (LFD) group (n=28), which was fed with low-fat diet as the control. After 16 weeks on diet, each diet group was divided into periodontitis (P) and control (C) groups. The P groups were induced for periodontitis by ligation with Porphyromonas gingivalis-adhered silk for 5 or 10 days, and the C groups were sham-ligated as the control. Visceral organs were resected and weighed. The organ weight percentage was calculated. Results Compared with the LFD group, the HFD group significantly upregulated the weight and weight percentage of visceral adipose tissue and spleen (P<0.05), upregulated the weight of liver and kidney (P<0.05), and down-regulated the weight percentage of liver and kidney (P<0.01). In the HFD group, the weight and weight percentage of spleen were downregulated in the P group (P<0.05), but were up-regulated in the 10-day group compared with the 5-day group (P<0.05). Conclusion Periodontitis can affect the general morphology of the viscera (especially spleen) in obese animal models. Pathological indications in terms of immunometabo-lism might be present in the correlation between obesity and periodontitis.

Key words: obesity, periodontitis, viscera, spleen, pathology

CLC Number:

R781.4

Ting Yu,Li Zhao,Jincai Zhang,Dongying Xuan. Impacts of periodontitis on visceral organ weight and weight percentage in obese mice[J]. West China Journal of Stomatology, 2018, 36(5): 514-520.

Figures/Tables 5

Fig 1

Tab 1

Tab 2

Three-way statistical description on weight and weight percentages of visceral organs in different diet, ligation and ligation duration $\bar{x}$±SD

参数	结扎期	HFD		LFD
参数	结扎期	P组	C组	P组	C组
EWAT/g	5 d	1.132 2±0.268 7^M	1.131 2±0.193 7^BM	0.219 8±0.028 2^N	0.234 1±0.029 3^N
	10 d	1.022 0±0.252 1^M	0.881 4±0.173 9^AM	0.145 0±0.075 3^XN	0.229 3±0.042 3^YN
EWATp/%	5 d	2.83±0.51^M	2.81±0.27^BM	0.77±0.08^N	0.83±0.09^N
	10 d	2.71±0.45^YM	2.29±0.32^AXM	0.53±0.27^XN	0.82±0.18^YN
PWAT/g	5 d	0.506 4±0.171 2^M	0.506 9±0.156 0^BM	0.089 2±0.029 6^BN	0.085 9±0.014 0^N
	10 d	0.587 9±0.460 9^M	0.352 2±0.127 5^AM	0.048 8±0.027 0^AXN	0.102 5±0.042 9^YN
PWATp/%	5 d	1.25±0.34^M	1.24±0.24^BM	0.31±0.09^BN	0.30±0.03^N
	10 d	1.50±1.01^M	0.90±0.24^AM	0.18±0.09^AXN	0.37±0.17^YN
MWAT/g	5 d	1.149 4±0.356 5^M	1.261 7±0.458 7^BM	0.494 4±0.048 7^BN	0.447 1±0.121 0^N
	10 d	1.015 0±0.505 2^M	0.810 2±0.203 8^AM	0.256 6±0.062 1^AXN	0.350 7±0.075 7^YN
MWATp/%	5 d	2.91±0.69^M	3.08±0.78^BM	1.73±0.10^BN	1.58±0.37^N
	10 d	2.62±1.00^M	2.10±0.39^AM	0.98±0.20^AN	1.25±0.28^N
肝/g	5 d	1.705 2±0.481 3	1.770 4±0.749 9	1.384 0±0.136 5	1.365 9±0.214 9
	10 d	1.480 6±0.334 0	1.450 7±0.171 3^M	1.392 7±0.241 1	1.237 5±0.155 0^N
肝p/%	5 d	4.27±0.93	4.32±1.32	4.86±0.60	4.84±0.61
	10 d	3.99±0.96^N	3.79±0.32^N	5.30±0.65^YM	4.42±0.63^XM
脾/g	5 d	0.103 7±0.021 7	0.105 5±0.019 7^AM	0.100 3±0.020 2	0.086 6±0.012 3^N
	10 d	0.115 5±0.041 1^X	0.189 0±0.048 6^BYM	0.112 5±0.042 1	0.097 3±0.021 5^N
脾p/%	5 d	0.27±0.08^N	0.27±0.05^A	0.35±0.07^M	0.31±0.03
	10 d	0.32±0.15^X	0.50±0.15^BYM	0.43±0.16	0.34±0.07^N
肾/g	5 d	0.283 1±0.046 5^M	0.280 9±0.034 2^M	0.231 4±0.025 7^N	0.221 3±0.030 7^N
	10 d	0.255 4±0.031 4	0.288 5±0.039 8^M	0.229 0±0.035 2	0.215 7±0.017 5^N
肾p/%	5 d	0.72±0.09^N	0.70±0.05^N	0.81±0.07^M	0.78±0.07^M
	10 d	0.69±0.08^N	0.75±0.06	0.88±0.13^M	0.77±0.05

Tab 2

Tab 3

Tab 4

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参数	不控制主效应		控制主效应
参数	R值	P值	R值	P值
EWAT	0.927	<0.001	0.571	<0.001
EWATp	0.889	<0.001	0.219	0.093
PWAT	0.787	<0.001	0.482	<0.001
PWATp	0.772	<0.001	0.368	0.004
MWAT	0.847	<0.001	0.724	<0.001
MWATp	0.796	<0.001	0.605	<0.001
肝	0.533	<0.001	0.651	<0.001
肝p	-0.265	0.035	0.332	0.009
脾	0.239	0.057	-0.195	0.131
脾p	-0.207	0.100	-0.369	0.003
肾	0.765	<0.001	0.653	<0.001
肾p	-0.449	<0.001	0.066	0.614

参数	饲料		结扎		结扎期		合计
参数	HFD组	LFD组	P组	C组	5 d	10 d	合计
EWAT/g	1.041 7±0.239 6	0.209 3±0.056 0	0.703 1±0.489 8	0.667 4±0.423 6	0.735 9±0.486 4	0.632 5±0.419 4	0.685 0±0.454 0
EWATp/%	2.66±0.44	0.75±0.20	1.89±1.13	1.80±0.92	1.94±1.06	1.74±0.98	1.84±1.02
PWAT/g	0.488 3±0.268 3	0.081 6±0.035 0	0.337 9±0.349 2	0.282 8±0.207 2	0.323 3±0.242 2	0.297 5±0.327 7	0.310 4±0.286 2
PWATp/%	1.23±0.57	0.29±0.12	0.88±0.79	0.75±0.44	0.84±0.52	0.80±0.75	0.82±0.64
MWAT/g	1.056 5±0.418 6	0.383 2±0.120 8	0.769 7±0.489 9	0.757 2±0.450 2	0.888 2±0.483 0	0.646 2±0.423 9	0.763 3±0.466 0
MWATp/%	2.67±0.81	1.37±0.39	2.13±1.00	2.07±0.86	2.41±0.89	1.81±0.87	2.10±0.92
肝/g	1.601 7±0.483 1	1.345 0±0.191 7	1.503 4±0.348 4	1.475 4±0.455 2	1.579 0±0.501 5	1.399 8±0.246 7	1.489 4±0.402 3
肝p/%	4.10±0.94	4.86±0.67	4.55±0.93	4.31±0.88	4.54±0.94	4.31±0.87	4.43±0.91
脾/g	0.128 4±0.049 0	0.099 2±0.026 6	0.108 2±0.031 9	0.123 0±0.051 3	0.099 7±0.019 6	0.131 5±0.053 4	0.115 6±0.043 0
脾p/%	0.34±0.15	0.36±0.10	0.34±0.13	0.36±0.13	0.29±0.07	0.40±0.15	0.35±0.13
肾/g	0.277 0±0.038 9	0.224 3±0.027 2	0.252 2±0.041 0	0.255 7±0.045 6	0.257 7±0.044 4	0.250 2±0.042 0	0.253 9±0.043 0
肾p/%	0.71±0.07	0.81±0.09	0.76±0.11	0.75±0.06	0.75±0.08	0.76±0.10	0.76±0.09

参数	统计量	饲料	结扎	结扎期	饲料×结扎	饲料×结扎期	结扎×结扎期	饲料×结扎×结扎期
EWAT	F值	351.502	0.059	6.094	1.821	2.479	0.153	1.388
	P值	<0.001	0.810	0.017	0.183	0.121	0.697	0.244
EWATp	F值	549.513	0.071	7.400	5.999	1.434	0.272	3.745
	P值	<0.001	0.791	0.009	0.018	0.236	0.604	0.058
PWAT	F值	63.986	0.825	0.227	1.972	0.059	0.776	2.077
	P值	<0.000 1	0.368	0.635	0.166	0.808	0.382	0.155
PWATp	F值	75.595	0.978	0.124	3.437	0.004	0.830	3.342
	P值	<0.001	0.327	0.726	0.069	0.948	0.366	0.073
MWAT	F值	72.529	0.021	8.499	0.195	0.636	0.310	2.110
	P值	<0.001	0.886	0.005	0.661	0.428	0.580	0.152
MWATp	F值	71.504	0.137	14.856	0.588	0.106	0.186	3.407
	P值	<0.001	0.713	<0.001	0.447	0.746	0.668	0.070
肝	F值	6.951	0.125	2.907	0.287	1.188	0.355	0.012
	P值	0.011	0.725	0.094	0.594	0.280	0.554	0.915
肝p	F值	13.310	1.565	0.915	0.783	1.000	1.788	0.527
	P值	<0.001	0.216	0.343	0.380	0.322	0.187	0.471
脾	F值	13.452	2.112	13.743	10.704	5.139	4.835	5.266
	P值	<0.001	0.152	<0.001	0.002	0.027	0.032	0.026
脾p	F值	0.521	0.215	14.121	7.968	2.487	1.669	4.236
	P值	0.474	0.644	<0.001	0.007	0.120	0.202	0.044
肾	F值	36.965	0.048	0.663	2.455	0.122	0.852	1.243
	P值	<0.001	0.828	0.419	0.123	0.729	0.360	0.270
肾p	F值	24.120	1.035	0.845	5.591	0.149	0.001	4.246
	P值	<0.001	0.313	0.362	0.022	0.701	0.980	0.044