Effect of water immersion on a dental self-healing and antibacterial resin composite

doi:10.7518/hxkq.2018.05.011

Abstract

Abstract:

Objective This investigation aimed to develop a novel self-healing and antibacterial dental resin composite. The effects of water immersion on its properties were also evaluated. Methods Microcapsules filled with healing agent of triethylene glycol dimethacrylate were synthesized on the basis of previous studies. Antibacterial resin composite contained nano-antibacterial inorganic fillers that were modified by quaternary ammonium salt with long-chain alkyl. Microcapsules were incorporated into antibacterial resin composite at mass fraction of 7.5%. A commercial resin composite named Tetric N-Ceram was used as control. The resin samples were immersed in 37 °C distilled water for different periods. A flexural test was used to measure the mechanical properties of the novel resin composite. A single-edge V-notched beam method was used to measure fracture toughness and self-healing efficiency. A dental plaque microcosm biofilm model with human saliva as inoculum was formed. Colony-forming units (CFU) and lactic acid production of biofilm on the novel resin composite were calculated to test the antibacterial property. Results Mechanical properties and fracture toughness decreased significantly after the composite was immersed in water for 30 days (P<0.05), and no significant reduction was found from then on (P>0.05). Water immersion did not weaken the self-healing capability of the composite (P>0.05), and self-healing efficiency of 64% could still be obtained even after 270 days. The antibacterial resin composite showed a strong inhibition effect on the biofilm metabolic activity versus water immersion time from 1 day to 270 days. Therefore, the com-posite could still have a promising antibacterial property even after being immersed in water (P<0.05). Conclusion Water immersion could weaken the mechanical properties of the novel self-healing and antibacterial resin composite, but it insig-nificantly affected the self-healing and antibacterial properties of the composite.

Key words: water immersion, microcapsule, self-healing, antibacterial property, resin composite

CLC Number:

R783

Junling Wu,Tong Li,Xu Gao,Qiang Zhang,Di Liu,Jianhua Ge,Chuanjian Zhou. Effect of water immersion on a dental self-healing and antibacterial resin composite[J]. West China Journal of Stomatology, 2018, 36(5): 521-527.

Figures/Tables 5

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组别	观测时间/d	弯曲强度/MPa	弹性模量/GPa
对照组	1	100±16^a	6.2±0.9^a
	30	69±12^b	4.3±0.7^b
	90	70±11^b	4.3±0.7^b
	180	70±10^b	4.2±0.6^b
	270	69±11^b	4.1±0.7^b
实验组	1	95±13^a	6.1±1.1^a
	30	67±11^b	4.0±0.8^b
	90	69±10^b	4.1±0.7^b
	180	70±8^b	4.3±0.7^b
	270	70±12^b	4.2±0.8^b

组别	观测时	K_IC-virgin/	K_IC-healed/	自修复效
组别	间/d	（MPa·m^1/2）	（MPa·m^1/2）	率η/%
对照组	1	1.2±0.2^a	0	0
	30	0.8±0.2^b	0	0
	90	0.8±0.1^b	0	0
	180	0.7±0.1^b	0	0
	270	0.7±0.1^b	0	0
实验组	1	1.2±0.1^a	0.8±0.1^a	68±13^a
	30	0.8±0.1^b	0.5±0.1^b	66±10^a
	90	0.7±0.1^b	0.5±0.1^b	69±10^a
	180	0.7±0.1^b	0.5±0.1^b	67±8^a
	270	0.7±0.1^b	0.5±0.1^b	64±8^a

组别	观测时间/d	菌落计数/CFU			乳酸量/mmol·L^-1
组别	观测时间/d	全菌落	总链球菌	变异链球菌	乳酸量/mmol·L^-1
对照组	1	（5.7 ±1.0）×10^{9 a}	（5.9 ±1.1）×10^{8 a}	（6.0 ±1.1）×10^{7 a}	22.5±2.8 ^a
	30	（5.6 ±1.0）×10^{9 a}	（5.8 ±1.0）×10^{8 a}	（6.1 ±1.2）×10^{7 a}	23.7±4.1 ^a
	90	（6.2 ±1.3）×10^{9 a}	（5.6 ±1.0）×10^{8 a}	（5.9 ±1.0）×10^{7 a}	21.6±3.1 ^a
	180	（6.1 ±1.1）×10^{9 a}	（5.7 ±1.0）×10^{8 a}	（6.3 ±1.0）×10^{7 a}	23.1±3.2 ^a
	270	（5.8 ±1.1）×10^{9 a}	（5.9 ±1.2）×10^{8 a}	（6.0 ±1.0）×10^{7 a}	23.9±4.0 ^a
实验组	1	（5.7 ±1.0）×10^{6 b}	（5.7 ±1.0）×10^{5 b}	（5.8 ±1.2）×10^{3 b}	0.23±0.04 ^b
	30	（5.8 ±1.1）×10^{6 b}	（5.6 ±1.0）×10^{5 b}	（6.2 ±1.1）×10^{3 b}	0.22±0.03 ^b
	90	（5.7 ±1.0）×10^{6 b}	（6.0 ±1.0）×10^{5 b}	（6.0 ±1.0）×10^{3 b}	0.26±0.04 ^b
	180	（6.0 ±1.1）×10^{6 b}	（6.1 ±1.1）×10^{5 b}	（6.2 ±1.2）×10^{3 b}	0.25±0.04 ^b
	270	（6.3 ±1.1）×10^{6 b}	（6.1 ±1.2）×10^{5 b}	（6.3 ±1.2）×10^{3 b}	0.24±0.04 ^b