West China Journal of Stomatology ›› 2025, Vol. 43 ›› Issue (1): 46-52.doi: 10.7518/hxkq.2024.2024162
• Basic Research • Previous Articles Next Articles
Fu Lanqing(), Hao Xinyu, Qian Wenbo, Sun Ying(
)
Received:
2024-04-24
Revised:
2024-08-06
Online:
2025-02-01
Published:
2025-01-22
Contact:
Sun Ying
E-mail:2577334820@qq.com;ebolasun@njmu.edu.cn
Supported by:
CLC Number:
Fu Lanqing, Hao Xinyu, Qian Wenbo, Sun Ying. Effects of initial periodontal therapy on the formation of neutrophil extracellular traps in gingival crevicular fluid in patients with severe periodontitis[J]. West China Journal of Stomatology, 2025, 43(1): 46-52.
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Tab 1
Sequences of probes and primers for PCR
项目 | 引物序列(5’-3’) | 产物大小/bp |
---|---|---|
总菌 | FJ:AGAGTTTGATCCTGGCTCAG RJ:ACGGCTACCTTGTTACGACTT F:TCCTACGGGAGGCAGCAGT R:GGACTACCAGGGTATCTAATCCTGTT P:FAM-CGTATTACCGCGGCTGCTGGCAC-MGB | 1 495 462 |
P. gingivalis | FJ:CAGCTTGCCATACTGCG RJ:ACTGTTAGCAACTACCGATGT F:TACCCATCGTCGCCTTGGT R:CGGACTAAAACCGCATACACTTG P:FAM-ATTTATAGCTGTAAGATAGGC-MGB | 404 102 |
A. actinomycetemcomitans | FJ:ATGCCAACTTGACGTTAAAT RJ:AAACCCATCTCTGAGTTCTTCTTC F:TTGATCGTGCGAGAATGCTT R:ATCGCCGTTATAACCAAATTTCTT P:FAM-AGGAATACTCGAAACGC-MGB | 551 65 |
P. intermedia | FJ:TTTGTTGGGGAGTAAAGCGGG RJ:TCAACATCTCTGTATCCTGCGT F:TCCACCGATGAATCTTTGGTC R:ATCCAACCTTCCCTCCACTC P:FAM-CGTCAGATGCCATATGTGGACAACATCG-MGB | 575 103 |
Tab 3
Correlation analysis between NET levels and periodontal parameters, levels of TNF-α, IL-8 and quantities of total bacteria, P. gingivalis, A. actinomycetemcomitans and P. intermedia
指标 | r | P值 |
---|---|---|
PLI | 0.608 | 0.012* |
GI | 0.822 | 0.023* |
PD | 0.886 | 0.019* |
CAL | 0.893 | 0.007** |
TNF-α | 0.833 | 0.01* |
IL-8 | 0.800 | 0.009** |
总菌 | 0.748 | 0.005** |
P. gingivalis | 0.718 | 0.006** |
A. actinomycetemcomitans | 0.619 | 0.102 |
P. intermedia | 0.143 | 0.76 |
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