West China Journal of Stomatology ›› 2020, Vol. 38 ›› Issue (3): 297-300.doi: 10.7518/hxkq.2020.03.012

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Improvement program on pretreatment of acid decalcified tissue in hematoxylin-eosin staining

Yao Lihong, Zhang Mei, Huang Meichang, Wan Zixin, Zhang Weilong, Yang Xiao, Yang Mingzhong, Chen Yu, Tang Yaling()   

  1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Pathology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2019-09-22 Revised:2020-02-04 Online:2020-06-01 Published:2020-05-28
  • Contact: Yaling Tang E-mail:tangyaling@scu.edu.cn
  • Supported by:
    Clinical Research Project of West China Hospital of Stomatology, Sichuan University(LCYJ2019-8)


Objective To explore the treatment conditions of acid decalcified specimens and improve the poor quality of sections and unclear structure of hematoxylin-eosin (HE) staining caused by the change in pH in tooth and hard tissue after acid decalcification. Methods A total of 20 cases of oral pathological specimens that contain hard tissues were decalcified and treated with routine treatment, concentrated ammonia water immersion treatment, and saturated lithium carbonate solution immersion treatment. The quality and HE staining effects of hard tissue sections treated with different methods were compared. Results Compared with routine treatment, lithium carbonate saturated solution treatment showed complete sections. Hematoxylin is strongly stained, the nucleus is clear, and the cytoplasm is bright. Conclusion Soaking acid decalcified specimens in lithium carbonate saturated solution before embedding in dehydration can neutralize the acidic environment of the tissue. The quality of sections and HE staining effect are improved and are suitable for the pretreatment of acid decalcified tissue samples of oral pathology.

Key words: acid decalcification specimens, hematoxylin-eosin staining, concentrated ammonia water, saturated lithium carbonate solution

CLC Number: