Effect of 17% ethylenediaminetetraacetic acid irrigation on the dentin adhesion of Enterococcus faecalis

doi:10.7518/hxkq.2019.04.006

Abstract

Abstract:

Objective This work aims to uncover the promoting effect of 17% ethylenediaminetetraacetic acid (EDTA) irrigation on the dentin adhesion of Enterococcus faecalis (E. faecalis). Methods Forty-eight half split samples and twelve dentin slices were randomly divided into three experimental groups and one control group. The experimental groups and the control group were soaked with EDTA in different time lengths and with normal saline, respectively. E. faecalis was inoculated, and its dentin adhesion was measured via scanning electron microscopy, confocal laser scanning microscopy (CLSM), colony forming unit counts, and histological Gram staining. Results According to histological Gram staining, the depth showed no statistically significant differences between 1 min group and the control group, 1 min group and 3 min groups (P>0.05). E. faecalis intruded in the dentine tubules (measured by CLSM), and the thickness of the biofilm on the dentin surface and the colony numbers of experimental groups were greater than those of the control group (P<0.05). The differences between the three experimental groups were statistically significant (P<0.05). Conclusion EDTA (17%) irrigation can promote E. faecalis adhesion to dentin. This adhesion would in turn prolong EDTA treatment time.

Key words: ethylenediaminetetraacetic acid, dentin, Enterococcus faecalis, adhesion, infection

CLC Number:

R780.2

Xiaoxia Shen,Yu Sun,Lijun Huo,Rui She,Yayan Lei. Effect of 17% ethylenediaminetetraacetic acid irrigation on the dentin adhesion of Enterococcus faecalis[J]. West China Journal of Stomatology, 2019, 37(4): 372-377.

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References 10

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组别	菌落数/ ( lxlOSCFI^mL-¹)
对照	7.667±0.250
1 min	27.000±1.557*
3 min	51.000±2.550*^#
5 min	106.333±6.317*^#A

组别	生物膜厚度
对照	10.330t0.595
1 min	14.367士0.468*
3 min	20.897±1.165*^#
5 min	26.767±1.116*^#A

组别	侵入深度
对照	758.327±81.002
1 min	1 208.033±69.236*
3 min	1 560.030±41.994*^#
5 min	2 041.353±69.600*^#A

组别	侵入深度
对照	234.875±16.514
1 min	604.414士 65.318
3 min	908.308±35.091*
5 min	1 536.528±50.719*^#A

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