Oral microbiological diversity in patients with salivary adenoid cystic carcinoma

doi:10.7518/hxkq.2019.03.015

Abstract

Abstract:

Objective The aim of this study was to identify the differences in microbial diversity and community in patients with salivary adenoid cystic carcinoma (SACC). Methods Saliva was collected from 13 patients with SACC confirmed by histopathological diagnosis and 10 healthy control subjects. Total metagenomic DNA was extracted. The DNA amplicons of the V3-V4 hypervariable regions of the 16S rRNA gene were generated and subjected to high-throughput sequencing. Microbial diversity and community structure were analyzed with Mothur software. Results A total of 16 genera of dominant bacteria in the SACC group were found, including Streptococcus (36.68%), Neisseria (8.55%), Prevotella_7 (7.53%), and Veillonella (6.37%), whereas 15 dominant bacteria in the control group were found, including Streptococcus (18.41%), Neisseria (18.20%), Prevotella-7 (8.89%), Porphyromonas (6.20%), Fusobacterium (5.86%) and Veillonella (5.82%). The statistically different phyla between the two groups were Firmicutes, Proteobacteria and Fusobacterium (P<0.05). The statistically different genera between the two groups were Streptococcus, Neisseria andPorphyromonas (P<0.05), and Capnocytophaga was only detected in patients with SACC. Conclusion Significant differences were observed in the oral microorganisms between the two groups.

Key words: salivary adenoid cystic carcinoma, high-throughput sequencing, saliva, microbial diversity

CLC Number:

R780.2

Xing Liu,Qifen Yang,Ning Gan,Deqin Yang. Oral microbiological diversity in patients with salivary adenoid cystic carcinoma[J]. West China Journal of Stomatology, 2019, 37(3): 304-308.

Figures/Tables 3

References 23

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