关键词: 透明质酸, 胶原, 牙周膜细胞, 支架材料

Abstract:

Objective The adhesion and growth of human periodontal ligament cells（PDLC） on collagen（Col）, hyaluronic acid （HA） and HA/Col scaffolds were studied to evaluate the feasibility of HA/Col as a scaffold matetial in periodontal tissue engineering. Methods Human PDLC cultured in vitro was collected and seeded on Col, HA, HA/Col scaffolds crosslinked by carbodiimide. The influences of scaffolds on cell adhesion and growth were observed by MTT assay. The growth of human PDLC on scaffolds was observed through inverted phase contrast microscope and scanning electron microscope（SEM）. Results The result of MTT assay demonstrated that except the seventh day there was obviously differentiation between each group Col, HA, HA/Col scaffolds at the first, second and fourth day, and HA/Col group was higher than control group all the time. Human PDLC successfully grew on scaffolds. Conclusion HA/Col scaffold was more suitable for human PDLC adhesion compared with Col scaffold and HA scaffold. This indicates that HA/Col scaffold might be a potential ideal candidate for periodontal tissue engineering.

Key words: hyaluronic acid, collagen, periodontal ligament cells, scaffold