碱性成纤维细胞生长因子/壳聚糖衍生物/胶原温敏型复合水凝胶促大鼠牙周组织再生的研究

doi:10.7518/hxkq.2023.01.003

华西口腔医学杂志 ›› 2023, Vol. 41 ›› Issue (1): 21-28.doi: 10.7518/hxkq.2023.01.003

碱性成纤维细胞生长因子/壳聚糖衍生物/胶原温敏型复合水凝胶促大鼠牙周组织再生的研究

谭仲娟¹(), 罗圆圆², 杨莉¹()

^1.广州医科大学附属口腔医院牙体牙髓科·广东省口腔组织修复与重建工程技术研究中心· 广州市口腔再生医学基础与应用研究重点实验室，广州 510182
^2.聊城市人民医院口腔科，聊城 252020

收稿日期:2022-07-21 修回日期:2022-12-26 出版日期:2023-02-01 发布日期:2023-02-21
通讯作者: 杨莉 E-mail:791074070@qq.com;2382859867@qq.com
作者简介:谭仲娟，医师，硕士，E-mail：791074070@qq.com
基金资助:
广州市科技计划项目(20180310044)

Basic fibroblast growth factor/chitosan derivatives/collagen composite thermosensitive hydrogel promotes perio-dontal tissue regeneration in rats

Tan Zhongjuan¹(), Luo Yuanyuan², Yang Li¹()

^1.Dept. of Endodontics, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou 510182, China
^2.Dept. of Stomatology, Liaocheng People’s Hospital, Liaocheng 252020, China

Received:2022-07-21 Revised:2022-12-26 Online:2023-02-01 Published:2023-02-21
Contact: Yang Li E-mail:791074070@qq.com;2382859867@qq.com
Supported by:
Technology Program Key Projects of Guangzhou(201803010044)

摘要/Abstract

摘要：

目的探讨不同种壳聚糖衍生物温敏型复合水凝胶作为支架材料应用于牙周组织工程的可行性。方法制备磺化壳聚糖（SCS）、磷酸化壳聚糖（PCS）及磷酸化磺化壳聚糖（PSCS）3种具有不同生物学特性的壳聚糖衍生物，构建3种碱性成纤维细胞生长因子（bFGF）/壳聚糖衍生物/胶原温敏型复合水凝胶。选取20只雄性wistar大鼠，大鼠双侧上颌第一磨牙近中建立三壁骨袋，随机分为空白对照组、空白凝胶组、bFGF/SCS/胶原温敏型复合水凝胶组、bFGF/PCS/胶原温敏型复合水凝胶组、bFGF/PSCS/胶原温敏型复合水凝胶组，术后6周处死大鼠，采集标本，进行大体、苏木精-伊红染色、Masson染色观察。结果术后6周，3种bFGF/壳聚糖衍生物/胶原温敏型复合水凝胶组与空白对照组在相对牙槽骨高度比值、相对上皮根向下移比及牙周组织再生分级计数等方面的差异均具有统计学意义（P<0.05）。结论 bFGF/壳聚糖衍生物/胶原温敏型复合水凝胶在牙周组织工程邻域具有良好的应用前景。

关键词: 水凝胶, 壳聚糖衍生物, 碱性成纤维生长因子, 胶原, 牙周组织, 再生

Abstract:

Objective To investigate the feasibility of different thermosensitive composite hydrogels from chitosan derivatives as scaffold materials for periodontal tissue engineering. Methods Three chitosan derivatives with different biological characteristics were prepared, namely, sulfonated chitosan (SCS), phosphorylated chitosan (PCS), and phosphorylated sulfonated chitosan (PSCS). Three thermosensitive composite hydrogels were constructed using basic fibroblast growth factor (bFGF), the chitosan derivatives, and collagen. Twenty male Wistar rats were randomly divided into control group, blank group, bFGF/SCS/collagen composite thermosensitive hydrogel group, bFGF/PCS/collagen compo-site thermosensitive hydrogel group, and bFGF/PSCS/collagen composite thermosensitive hydrogel group. Then, three-wall intrabony defects were established. The defects were treated with the different kinds of thermosensitive composite hydrogels. After 6 weeks of surgery, the animals were killed, and specimens were collected. Then, gross observation, hematoxylin-eosin staining, and Masson staining were performed. Results The bFGF/chitosan derivatives/collagen composite thermosensitive hydrogel groups and the control group had statistical differences in the relative alveolar bone height, relative epithelial down growth and grading count score of periodontal tissue regeneration (P<0.05). Conclusion bFGF/chitosan derivatives/collagen composite thermosensitive hydrogels have good application prospects in periodontal tissue engineering.

Key words: hydrogel, chitosan derivatives, basic fibroblast growth factor, collagen, periodontal tissue, regeneration

中图分类号:

R 781.4

谭仲娟, 罗圆圆, 杨莉. 碱性成纤维细胞生长因子/壳聚糖衍生物/胶原温敏型复合水凝胶促大鼠牙周组织再生的研究[J]. 华西口腔医学杂志, 2023, 41(1): 21-28.

Tan Zhongjuan, Luo Yuanyuan, Yang Li. Basic fibroblast growth factor/chitosan derivatives/collagen composite thermosensitive hydrogel promotes perio-dontal tissue regeneration in rats[J]. West China Journal of Stomatology, 2023, 41(1): 21-28.

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参考文献 22

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级别	描述
0	没有PDL的再生及CT附着，纤维无序，上皮根向下移
1	没有PDL再生，但有CT附着及垂直方向的纤维形成
2	在缺损上方有PDL再生及垂直方向的纤维形成
3	完全的PDL再生

样品	元素分析					磺化率	磷酸化率
样品	C	H	N	S	P	磺化率	磷酸化率
CS	40.82	7.22	7.35	-	-	-	-
SCS	20.39	4.12	3.99	12.66	-	138.82	-
PCS	32.15	6.56	5.78	-	5.69	-	44.33
PSCS	24	5.27	4.37	12.15	0.34	121.64	3.5

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碱性成纤维细胞生长因子/壳聚糖衍生物/胶原温敏型复合水凝胶促大鼠牙周组织再生的研究

Basic fibroblast growth factor/chitosan derivatives/collagen composite thermosensitive hydrogel promotes perio-dontal tissue regeneration in rats

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