华西口腔医学杂志

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人乳牙牙根吸收过程中根面破骨细胞的观察

包向军1,梁 星2,陈 明2,王 航2,谢志刚2,杨晓喻2   

  1. 1.上海市口腔病防治院 修复科,上海200001;2.四川大学华西口腔医院 修复科,四川 成都610041
  • 收稿日期:2004-08-25 修回日期:2004-08-25 出版日期:2004-08-20 发布日期:2004-08-20
  • 通讯作者: 梁 星,Tel:028-85449115
  • 作者简介:包向军(1970-),男,辽宁人,主治医师,博士
  • 基金资助:

    国家自然科学基金资助项目(30271428);教育部“优秀青年教师资助计划”项目(教人司[2002]40号);高等学校博士学科点专项科研基金资助项目(B1200058)

Observation of Osteoclasts on the Root Surface during Human Deciduous Teeth Resorption

BAO Xiang-jun1,LIANG Xing2,CHENMing2,WANGHang2,XIE Zhi-gang2,YANGXiao-yu2   

  1. 1.Dept.ofProsthetics,Shanghai Preventive Hospital of Oral Diseases,Shanghai200001,China; 2.Dept.ofProsthetics,West China College ofStomatology,Sichuan University,Cheng- du610041,China
  • Received:2004-08-25 Revised:2004-08-25 Online:2004-08-20 Published:2004-08-20

摘要:

目的 观察人乳牙根吸收面的破骨细胞形态特点。方法 收集和固定人滞留乳牙,制备硬组织切片,经苏木精-伊红、抗酒石酸酸性磷酸酶染色,在光镜和扫描电镜下观察破骨细胞。结果 在人乳牙根吸收面有多个破骨细胞,呈重叠状态,细胞形态不规则,体积大,细胞周缘可见丝状突起,多核,被吸收的牙本质表面高低不平。结论 人滞留乳牙牙根吸收面存在大量的破骨细胞,是研究人破骨细胞的一个来源。

关键词: 乳牙, 破骨细胞, 形态学

Abstract:

Objective To observe osteoclasts on the resorbing surface of human deciduous teeth.Methods After fixing the collected deciduous teeth, we prepared the tooth sliceswithout decalcification, treated themwithHE andTRAP dyestuff, and ob- served the osteoclasts under light and scanning electronmicroscope.Results Therewere large quantity of various forms of overlap- ping and huge osteoclastswith many nuclei and silk-like protuberances on the resorbing surface of deciduous teeth. The multinu- cleated osteoclasts align on the surface of coarse dentin.Conclusion On the resorbing surface of human deciduous teeth there are large amount of osteoclasts which can be used as a source of studying human osteoclast.

Key words: deciduous teeth, osteoclasts, morphology