破骨细胞分化在颞下颌关节骨关节炎发生中的作用

doi:10.7518/hxkq.2021.04.004

华西口腔医学杂志 ›› 2021, Vol. 39 ›› Issue (4): 398-404.doi: 10.7518/hxkq.2021.04.004

破骨细胞分化在颞下颌关节骨关节炎发生中的作用

肖勉(), 胡智慧, 江恒华, 房维, 龙星()

武汉大学口腔医院口腔颌面创伤与颞下颌关节外科，湖北省口腔基础医学重点实验室－省部共建国家重点实验室培育基地，口腔生物医学教育部重点实验室（武汉大学），武汉 430000

收稿日期:2020-07-14 修回日期:2021-04-17 出版日期:2021-08-01 发布日期:2021-08-10
通讯作者: 龙星 E-mail:mingshore@whu.edu.cn;longxing@whu.edu.cn
作者简介:肖勉，硕士，E-mail：mingshore@whu.edu.cn
基金资助:
国家自然科学基金(81771100)

Role of osteoclast differentiation in the occurrence of osteoarthritis of temporomandibular joint

Xiao Mian(), Hu Zhihui, Jiang Henghua, Fang Wei, Long Xing()

Dept. of Oral and Maxillofacial Trauma and Temporomandibular Joint Surgery, School and Hospital of Stomatology, Wuhan University, State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM), School and Hospital of Stomatology, Wuhan University, Wuhan 430000, China

Received:2020-07-14 Revised:2021-04-17 Online:2021-08-01 Published:2021-08-10
Contact: Long Xing E-mail:mingshore@whu.edu.cn;longxing@whu.edu.cn
Supported by:
The National Natural Science Foundation of China(81771100)

摘要/Abstract

摘要： 目的

探究破骨细胞分化在颞下颌关节骨关节炎（TMJOA）发生中的作用。

方法

构建小鼠TMJOA模型，Micro-CT观察TMJOA发生发展过程中髁突骨质变化，苏木素伊红（HE）染色观察TMJOA关节组织学结构变化，抗酒石酸酸性磷酸酶（TRAP）组织染色观察TMJOA关节组织中破骨细胞的存在情况。收集TMJOA患者滑液，检测其对破骨细胞分化的作用。

结果

Micro-CT观察到TMJOA小鼠髁突在第2周和第3周时破坏最明显，形态也发生鸟嘴样改变；HE染色观察到TMJOA小鼠在第2周髁突软骨及软骨下骨结构紊乱；TRAP组织染色显示第2周时关节髁突中破骨细胞数量明显增多；细胞实验结果显示TMJOA患者滑液刺激后的破骨细胞分化数量显著增多，细胞体积变大。

结论

TMJOA动物模型及TMJOA患者滑液细胞实验均可诱导破骨细胞分化，表明破骨细胞分化在颞下颌关节骨关节炎的发生中起重要作用。

关键词: 颞下颌关节骨关节炎, 滑液, 破骨细胞, 髁突, 破骨分化

Abstract: Objective

This study aimed to explore the role of osteoclast differentiation in the occurrence of temporomandibular joint osteoarthritis (TMJOA).

Methods

A mouse TMJOA model was constructed. Micro-CT was used to observe the changes in condylar bone during the development of TMJOA. Hematoxylin-eosin (HE) staining was used to observe the histological structure changes of the condyle of TMJOA mice. Tartrate resistant acid phosphatase (TRAP) staining was used to observe the presence of osteoclasts in TMJOA joint tissue. The synovial fluid of patients with TMJ-OA was collected to determine the effect on osteoclast differentiation.

Results

Micro-CT revealed that the condyle of the TMJOA group had the most obvious damage in the second and third weeks, and the shape of the condyles also changed in a beak-like manner. HE staining showed that the condyle cartilage and subchondral bone structure of TMJOA mice were disordered in the second week. TRAP tissue staining showed that the number of osteoclasts of the TMJOA group obviously increased in the second week. Results of cell experiments showed that the number of osteoclast differentiation significantly increased after stimulation of synovial fluid from TMJOA patients, and the cell volume increased.

Conclusion

TMJOA animal models and TMJOA patient synovial cell experiments could induce osteoclast differentiation, indicating that osteoclast differentiation plays an important role in TMJOA occurrence.

Key words: temporomandibular joint osteoarthritis, synovial fluid, osteoclast, condyle, osteoclast differentiation

中图分类号:

R 782.6

肖勉, 胡智慧, 江恒华, 房维, 龙星. 破骨细胞分化在颞下颌关节骨关节炎发生中的作用[J]. 华西口腔医学杂志, 2021, 39(4): 398-404.

Xiao Mian, Hu Zhihui, Jiang Henghua, Fang Wei, Long Xing. Role of osteoclast differentiation in the occurrence of osteoarthritis of temporomandibular joint[J]. West China Journal of Stomatology, 2021, 39(4): 398-404.

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破骨细胞分化在颞下颌关节骨关节炎发生中的作用

Role of osteoclast differentiation in the occurrence of osteoarthritis of temporomandibular joint

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