双氢青蒿素抑制口腔鳞状细胞癌进展的研究

doi:10.7518/hxkq.2026.2025163

摘要/Abstract

摘要：

目的通过研究双氢青蒿素（DHA）对口腔鳞状细胞癌（OSCC）细胞系增殖、迁移和侵袭等生物学行为的影响，为后续研发新型药物治疗OSCC提供理论依据。方法通过癌症基因组学数据库进行生物信息学分析，以肿瘤组织是否存在囊外扩散转移对OSCC患者进行分类，比较不同OSCC细胞生存率的差异。CCK-8细胞毒性实验检测不同OSCC细胞系（CAL27、HN30和SCC9）经不同浓度DHA处理后的细胞增殖情况，进而筛选出最适药物浓度。CCK-8细胞增殖实验、细胞克隆实验检测DHA对OSCC细胞增殖能力的影响。划痕实验、Transwell实验检测DHA对肿瘤细胞迁移侵袭能力的影响；构建裸鼠皮下移植瘤模型，研究DHA对OSCC肿瘤组织及主要脏器组织的影响。结果生物信息学分析表明，肿瘤组织存在囊外扩散转移的患者的生存率低于无囊外扩散转移的患者（P<0.05）。CCK-8细胞毒性检测显示，DHA对CAL27、HN30和SCC9细胞的最大安全浓度分别为20、10和5 μmol/L。CCK-8细胞增殖实验、细胞克隆实验表明，DHA可以显著抑制CAL27、HN30和SCC9的增殖能力（P<0.05）；划痕实验、Transwell实验表明，DHA作用下CAL27、HN30和SCC9细胞的迁移和侵袭能力显著下降（P<0.05）。体内实验表明，经DHA干预后肿瘤组织生长速率显著降低（P<0.001）。结论 DHA对OSCC细胞的增殖、迁移、侵袭能力及肿瘤组织的进展具有显著的抑制作用。

关键词: 口腔鳞状细胞癌, 双氢青蒿素, 细胞增殖, 迁移, 侵袭

Abstract:

Objective This study aimed to explore the impact of dihydroartemisinin (DHA) on cell proliferation, migration, and invasion in oral squamous cell carcinoma (OSCC). Our findings offer a theoretical foundation for advancing the research and development of novel therapeutic agents for OSCC. Methods Bioinformatics analysis was conducted using the cancer genome database to classify OSCC patients based on the presence or absence of extracapsular spread and metastasis in tumor tissues, and to compare the differences in survival rates among different OSCC cell lines. The cell proliferation of different OSCC cell lines treated with different DHA concentrations was detected by CCK-8 cytoto-xicity assay, and the optimal drug concentration was screened. The CCK-8 cytotoxicity assay was used to detect the cell proliferation of different OSCC cell lines (CAL27, HN30, and SCC9) after treatment with different concentrations of DHA, and to screen out the optimal drug concentration. The effects of DHA on the proliferation of OSCC cells were detected by CCK-8 cell proliferation assay and cell cloning assay. The effect of DHA on the migration and invasion ability of tumor cells was detected by scratch test and Transwell test. A nude mouse subcutaneous tumor model was constructed to study the effects of DHA on OSCC tumor tissues and major organ. Results Bioinforma-tics analysis showed that the survival rate of patients with extracapsular spread and metastasis in tumor tissues was lower than that of patients without such phenomena (P<0.05). According to CCK-8 cytotoxicity results, the maximum safe concentrations for different OSCC cell lines CAL27, HN30, and SCC9 were 20, 10, and 5 μmol/L, respectively. Proliferation and cloning experiments of CCK-8 cells showed that DHA could inhibit the proliferation of three squamous cell lines (P<0.05). Scratch test and Transwell test showed that the migration and invasion levels of CAL27, HN30, and SCC9 cells were significantly decreased under DHA treatment (P<0.05). In vivo experiments showed that the growth rate of tumor tissue significantly decreased after DHA treatment (P<0.05). Conclusion DHA exhibits significant inhibitory effects on the proliferation, migration, and invasion of various OSCC cells and growth of tumor tissues.

Key words: oral squamous cell carcinoma, dihydroartemisinin, cell proliferation, migration, invasion

中图分类号:

R739.8

单天宇, 刘伽伽, 刘唐君, 孙丹丹, 王馨伟, 柳云霞. 双氢青蒿素抑制口腔鳞状细胞癌进展的研究[J]. 华西口腔医学杂志, 2026, 44(2): 224-231.

Shan Tianyu, Liu Jiajia, Liu Tangjun, Sun Dandan, Wang Xinwei, Liu Yunxia. Dihydroartemisinin inhibits the progression of oral squamous cell carcinoma[J]. West China Journal of Stomatology, 2026, 44(2): 224-231.

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