高尔基体膜蛋白1对口腔鳞状细胞癌细胞增殖、迁移和侵袭的影响及其机制

doi:10.7518/hxkq.2025.2025073

华西口腔医学杂志 ›› 2026, Vol. 44 ›› Issue (1): 82-93.doi: 10.7518/hxkq.2025.2025073

高尔基体膜蛋白1对口腔鳞状细胞癌细胞增殖、迁移和侵袭的影响及其机制

李首成¹^,²(), 文才²^,³, 余丽²^,⁴, 陈俊良²^,⁵, 冯浩¹^,²()

^1.西南医科大学附属口腔医院口腔颌面外科，泸州 646000
^2.西南医科大学口颌面修复重建和再生泸州市重点实验室，泸州 646000
^3.西南医科大学附属口腔医院修复科，泸州 646000
^4.西南医科大学附属口腔医院牙周黏膜病科，泸州 646000
^5.西南医科大学附属口腔医院综合急诊科，泸州 646000

收稿日期:2025-02-26 出版日期:2026-02-01 发布日期:2026-02-02
通讯作者: 冯浩 E-mail:1418497536@qq.com;fenghao@swmu.edu.cn
作者简介:李首成，医师，硕士，E-mail：1418497536@qq.com
基金资助:
四川省科学技术厅科技计划项目(2024JDRC0040)

Effect of Golgi membrane protein 1 on the proliferation, migration, and invasion of oral squamous cell carcinoma cells and its mechanism

Li Shoucheng¹^,²(), Wen Cai²^,³, Yu Li²^,⁴, Chen Junliang²^,⁵, Feng Hao¹^,²()

^1.Dept. of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou 646000, China
^2.Oral & Maxillofacial Reconstruction and Regeneration of Luzhou Key Laboratory, Luzhou 646000, China
^3.Dept. of Oral Implantology, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou 646000, China
^4.Dept. of Periodontics and Oral Mucosal Diseases, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou 646000, China
^5.Dept. of Comprehensive Emergency Medicine, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou 646000, China

Received:2025-02-26 Online:2026-02-01 Published:2026-02-02
Contact: Feng Hao E-mail:1418497536@qq.com;fenghao@swmu.edu.cn
Supported by:
Sichuan Science and Technology Program(2024JDRC0040)

摘要/Abstract

摘要：

目的探讨高尔基体膜蛋白1（GOLM1）在口腔鳞状细胞癌（OSCC）中的表达及其对OSCC细胞增殖、迁移、侵袭和上皮间充质转化（EMT）的影响和作用机制。方法利用生物信息学分析在TCGA数据库和GTEx数据库中有关头颈鳞状细胞癌（HNSCC）的数据，分析GOLM1在HNSCC中的表达情况及对其预后的影响。免疫组织化学法检测GOLM1在OSCC组织和癌旁组织中的表达情况。提取人正常口腔角质细胞（HOK）及OSCC细胞系HSC-3和SCC-25中GOLM1的mRNA和蛋白，利用荧光定量聚合酶链反应（qRT-PCR）和蛋白质免疫印记法（Western blot）检测GOLM1的表达差异。选取HSC-3和SCC-25进行体外实验，利用慢病毒转染沉默GOLM1，并通过qRT-PCR和Western blot验证转染效果；CCK-8和集落形成实验检测细胞增殖能力；划痕实验和Transwell实验检测细胞迁移和侵袭能力；Western blot检测EMT相关蛋白E-钙粘蛋白（E-cadherin）、N-钙粘蛋白（N-cadherin）和波形蛋白（Vimentin）的表达变化。基因集富集分析（GSEA）筛选GOLM1可能的作用通路，并通过Western blot和挽救实验进行验证。结果生物信息学分析结果发现GOLM1在HNSCC中高表达且与其预后不良有关。GOLM1在OSCC组织中高表达。GOLM1在OSCC细胞系HSC-3和SCC-25中的表达水平显著高于HOK。沉默GOLM1后，OSCC细胞的增殖、迁移和侵袭能力显著降低；E-cadherin的表达显著上升，而N-cadherin和Vimentin的表达显著下降。GSEA分析显示GOLM1与转化生长因子-β（TGF-β）信号通路密切相关；沉默GOLM1后，TGF-β1、Smad2和p-Smad2的表达降低；外源性重组人TGF-β1蛋白刺激可逆转沉默GOLM1导致的OSCC细胞增殖、迁移和侵袭能力降低。结论 GOLM1在OSCC组织和细胞中高表达，沉默GOLM1可通过TGF-β1/Smad2通路抑制OSCC细胞的增殖、迁移、侵袭和EMT。

关键词: 口腔鳞状细胞癌, 高尔基体膜蛋白1, 上皮间充质转化, 转化生长因子-β1/Smad2

Abstract:

Objective To investigate the expression of Golgi membrane protein 1 (GOLM1) in oral squamous cell carcinoma (OSCC) and its effects on proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) in OSCC cells and the underlying mechanisms. Methods Bioinformatics analysis was performed using the data from The Cancer Genome Atlas and Genotype-Tissue Expression databases to evaluate the expression of GOLM1 in head and neck squamous cell carcinoma (HNSCC) and its prognostic significance. Immunohistochemistry was used to detect GOLM1 expression in OSCC tissues and adjacent tissues. The mRNA and protein levels of GOLM1 in human normal oral keratinocytes (HOK) and OSCC cell lines (HSC-3 and SCC-25, respectively) were measured via real-time quantitative polymerase chain reaction (qRT-PCR) and Western blot. For in vitro experiments, GOLM1 was silenced in HSC-3 and SCC-25 cells via lentiviral transfection, with the transfection efficiency validated through qRT-PCR and Western blot. Cell proliferation was assessed through Cell Counting Kit-8 (CCK-8) and colony formation assays. Cell migration and invasion were evaluated via wound healing and Transwell assays, respectively. Western blot was used to analyze EMT-related proteins (E-cadherin, N-cadherin, and Vimentin). Gene set enrichment analysis (GSEA) was conducted to identify potential signaling pathways associated with GOLM1, followed by validation through Western blot and rescue experiments. Results GOLM1 exhibited a high expression in HNSCC, correlation with poor prognosis, and significant upregulation in OSCC tissues. In addition, GOLM1 showed markedly elevated expression levels in OSCC cell lines HSC-3 and SCC-25 compared with those in HOK cells. Silencing of GOLM1 markedly suppressed OSCC cell proliferation, migration, and invasion, accompanied with an increased E-cadherin expression and decreased N-cadherin and Vimentin levels. GSEA revealed a strong association between GOLM1 and the transforming growth factor-beta (TGF-β) signaling pathway. Silencing of GOLM1 reduced the expressions of TGF-β1, Smad2, and phosphorylated Smad2. Exogenous recombinant human TGF-β1 protein rescued the inhibitory effects of GOLM1 knockdown on OSCC cell proliferation, migration, and invasion. Conclusion GOLM1 is overexpressed in OSCC tissues and cells, and silencing of this protein inhibits OSCC cell proliferation, migration, invasion, and EMT via the TGF-β1/Smad2 signaling pathway.

Key words: oral squamous cell carcinoma, Golgi membrane protein 1, epithelial-mesenchymal transition, transforming growth factor-beta 1/Smad2

中图分类号:

R739.8

李首成, 文才, 余丽, 陈俊良, 冯浩. 高尔基体膜蛋白1对口腔鳞状细胞癌细胞增殖、迁移和侵袭的影响及其机制[J]. 华西口腔医学杂志, 2026, 44(1): 82-93.

Li Shoucheng, Wen Cai, Yu Li, Chen Junliang, Feng Hao. Effect of Golgi membrane protein 1 on the proliferation, migration, and invasion of oral squamous cell carcinoma cells and its mechanism[J]. West China Journal of Stomatology, 2026, 44(1): 82-93.

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基因名称	序列
sh-NC	CCTAAGGTTAAGTCGCCCTCG
sh-GOLM1 1#	GAACAGTGTGAGGAGCGAATA
sh-GOLM1 2#	GCAGGGAATGACAGAAACATA
sh-GOLM1 3#	CATCATCGTCTTGGGCTTCAA

基因名称	序列（5’-3’）
GOLM1	F：ATCACCACAGGTGAGAGGCTCA
GOLM1	R：ACTTCCTCTCCAGGTTGGTCTG
GAPDH	F：CAATGACCCCTTCATTGACC
GAPDH	R：GACAAGCTTCCCGTTCTCAG

高尔基体膜蛋白1对口腔鳞状细胞癌细胞增殖、迁移和侵袭的影响及其机制

Effect of Golgi membrane protein 1 on the proliferation, migration, and invasion of oral squamous cell carcinoma cells and its mechanism

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