干细胞局部应用对缺损面神经再生效果的系统评价

doi:10.7518/hxkq.2020.01.011

华西口腔医学杂志 ›› 2020, Vol. 38 ›› Issue (1): 59-68.doi: 10.7518/hxkq.2020.01.011

干细胞局部应用对缺损面神经再生效果的系统评价

赵丹,李月恒,杨正艳,蔡婷,吴晓艳,夏雨,周智()

重庆医科大学附属口腔医院预防口腔科口腔疾病与生物医学重庆市重点实验室重庆市高校市级口腔生物医学工程重点实验室,重庆 401120

收稿日期:2019-06-21 修回日期:2019-09-19 出版日期:2020-02-01 发布日期:2020-02-06
通讯作者: 周智 E-mail:500119@hospital.cqmu.edu.cn
作者简介:赵丹,硕士,E-mail： 2017110916@stu.cqmu.edu.cn
基金资助:
重庆高校创新团队建设计划(CXTDG201602006)

Effect of the local application of stem cells on repairing facial nerve defects: a systematic review

Zhao Dan,Li Yueheng,Yang Zhengyan,Cai Ting,Wu Xiaoyan,Xia Yu,Zhou Zhi()

Dept. of Preventive Stomatology, Stomatological Hospital of Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401120, China

Received:2019-06-21 Revised:2019-09-19 Online:2020-02-01 Published:2020-02-06
Contact: Zhi Zhou E-mail:500119@hospital.cqmu.edu.cn
Supported by:
Program for Innovation Team Building at Institutions of Higher Education in Chongqing(CXTDG201602006)

摘要/Abstract

摘要：

目的系统评价干细胞对面神经缺损的修复效果。方法在Pubmed、Cochrane Library、Web of Science、Embase、Scopus及中国生物医学文献数据库检索关于评价干细胞对动物面神经再生效果的所有原始研究,2名专业人员独立完成文献筛选、数据提取及偏倚风险评估。使用RevMan 5.3软件及随机效应模型进行统计分析,分析结果以均数差（MD）及95%可信区间（CI）的形式呈现。对面神经的功能性评估（胡须运动评分、面瘫评分）及组织学评估（有髓纤维密度、纤维直径、髓鞘厚度、G比值）结果进行Meta分析。结果从6个数据库共检索出4 614篇文献,15篇被纳入了Meta分析。干细胞组的胡须运动评分、面瘫评分、有髓纤维密度、髓鞘厚度均高于非干细胞组（P<0.05）,G比值小于非干细胞组（P=0.001）,纤维直径二者无统计学差异（P=0.08）。结论干细胞具有促进面神经再生的潜能。

关键词: 干细胞, 面神经, 神经组织工程, 系统评价

Abstract:

Objective To systematically evaluate the repairing effect of stem cells on facial nerve defects. Methods Articles regarding the regenerating effect of stem cells on facial nerves in animals were collected from the databases of Pubmed, Cochrane Library, Web of Science, Embase, Scopus, and CBM. Two professionals independently completed the article screening, data extraction, and bias risk assessment. RevMan 5.3 and random-effects models were used for the statistical analysis, and the results were presented in the form of mean differences (MD) with a 95%CI. The results of functional evaluation (vibrissae movement, facial paralysis) and histological evaluation (density of myelinated fibers, diameter of fibers, thickness of myelin sheath, G ratio) of facial nerve were Meta-analyzed. Results A total of 4 614 articles were retrieved from the 6 databases, and 15 of these articles were included in the Meta-analysis. For vibrissae movement and facial paralysis, the stem cell group scored significantly higher than the non-stem cell group (P<0.05). The density of myelinated fibers and thickness of the myelin sheath in the stem cell group were higher than those in the non-stem cell group (P<0.05). The G ratio in the stem cell group was smaller than that in the non-stem cell group (P=0.001). There was no significant difference in fiber diameter (P=0.08). Conclusion Stem cells have potential in promoting facial nerve regeneration.

Key words: stem cell, facial nerve, neural tissue engineering, systematic review

中图分类号:

赵丹,李月恒,杨正艳,蔡婷,吴晓艳,夏雨,周智. 干细胞局部应用对缺损面神经再生效果的系统评价[J]. 华西口腔医学杂志, 2020, 38(1): 59-68.

Zhao Dan,Li Yueheng,Yang Zhengyan,Cai Ting,Wu Xiaoyan,Xia Yu,Zhou Zhi. Effect of the local application of stem cells on repairing facial nerve defects: a systematic review[J]. West China Journal of Stomatology, 2020, 38(1): 59-68.

图/表 8

表 1

纳入研究的基本信息

作者及年份	动物种类	基线情况	总样	神经类型	间隙长	修复材料	细胞类型	细胞	随访
作者及年份	动物种类	基线情况	本量	神经类型	度/mm	或方法	细胞类型	剂量	时间
Abbas 2016^[24]	SD大鼠	雄性	12	面神经主干,		坐骨神经	ADSCs	1×10⁶	90 d
				颊支
Batioglu-Karaaltin	Wistar大鼠	雌性,350~380 g	27	面神经主干	2	导管	HOSCs	8×10⁶	10周
2016^[7]
Chen 2018^[25]	新西兰兔	7~8月,雄性,	45	颊支		导管	DPSCs	5×10⁶	10周
		（3.0±0.5） kg
Cho 2010^[26]	豚鼠		32	面神经主干	0	神经缝合	N-iHMSCs	1×10⁵	6周
Costa 2013^[27]	Wistar大鼠	成年,雄性,250~300 g	35	下颌缘支	5	PGA导管	BMSCs	4×10⁵	6周
Ghoreishian 2013^[28]	伊朗杂种犬	20~25 kg	7	颞支	>7	Gore-Tex导管	ADSCs	2×10⁷	12周
Guo 2009^[16]	家兔	任何性别,2~3 kg	36	颊支	10	壳聚糖导管	NSCs	1×10⁶	12周
Kwon 2009^[29]	SD大鼠		20	面神经主干	>5	硅胶导管	ADSCs		8周
Ma 2017^[30]	SD大鼠	成年,雌性,200~220 g	100	颊支	8	胶原导管	NS/PCs	1×10⁶	12周
Matsumine 2014^[31]	Lewis大鼠	8周,200~250 g	25	颊支	7	硅胶导管	DFATs	5×10⁴	13周
Matsumine 2017^[32]	Lewis大鼠	8周,雄性,200~250 g	30	颊支	7	硅胶导管	SVF	1×10³	13周
								1×10⁵
								1×10⁷
Salomone 2013^[33]	Wistar大鼠	雄性,250~300 g	48	下颌缘支	3	硅胶导管	BMSCs	4×10⁵	6周
Sasaki 2008^[18]	Lewis大鼠	8周	38	颊支	7	硅胶导管	DPSCs	1×10⁵	12 d
Sasaki 2011^[34]	Lewis大鼠	8周	10	颊支	7	PLGA导管	DPSCs	5×10⁵	5 d
Sasaki 2014^[17]	Lewis大鼠	成年,雄性	34	颊支	7	硅胶导管	DPSCs	1×10⁵	13周
Satar 2009^[35]	SD大鼠	12~16周,雌性,	15	颊支	0	神经缝合	BMSCs		6月
		220~228 g
Satar 2010^[36]	SD大鼠	12~16周,雌性,	3	颊支	0	神经缝合	BMSCs		9月
		220~228 g
Shi 2009^[5]	SD大鼠	成年,雌性,200~250 g	96	颊支	8	PLGA导管	NSCs,SCs	1×10⁵	12周
Shi 2012^[37]	SD大鼠	雌性,200~250 g	20	颊支	>5	PLGA导管	NSCs		12周
Shimizu 2018^[20]	Lewis大鼠	8周,雄性,200~250 g	24	颊支	7	PGA-胶原导管	ADSCs,SVF	1×10⁵	13周
Sun 2011(1)^[15]	SD大鼠	5~6周,雌性,100~150 g	40	颊支	8	去细胞动脉导管	ADSCs	1×10⁵	8周
Sun 2011(2)^[38]	SD大鼠	年轻,雌性,100~120 g	60	颊支	8	去细胞动脉导管	ADSCs,SCs	5×10⁵	8周
Sun 2018^[39]	新西兰兔	4月,雌性,3.0~3.5 kg	32	颊支	10	去细胞坐骨神经	ADSCs	2×10⁴	8周
Wang 2011^[40]	新西兰兔	成年,雌性,2.5~3.2 kg	54	颊支	10	自体静脉导管	BMSCs	3×10⁶	16周
Wang 2012^[41]	新西兰兔	任意性别,2.5~3.0 kg	16	颊支	7	硅胶导管	DPSCs	1×10⁴
Watanabe 2017^[42]	Lewis 大鼠	8周,雄性	77	颊支	7	硅胶导管	ADSCs,SCs	1×10⁵	13周
Yan 2004^[43]	新西兰兔	任意性别,2.0~3.0 kg	22	面神经主干	6	自体筋膜	NSCs		6周
Yu 2005^[44]	新西兰兔		15	颊支	>12	硅胶导管	BMSCs	1.6×10⁴	16周
Zhang 2008^[45]	新西兰兔	成年,2.0~2.5 kg	39	面神经主干	5	HA-胶原导管	NSCs	4×10⁶	12周
Zhang 2014^[46]	新西兰兔	成年,2.5~3.5 kg	20	颊支		硅胶导管	DPSCs		3月
Zhang 2018(1)^[47]	SD大鼠	成年,雌性,		颊支	6	AxoGuard导管	GMSCs,	5×10⁵	12周
		200~250 g					iNCSCs
Zhang 2018(2)^[19]	SD大鼠	8周,雌性,200~250 g		颊支	5	3D打印导管	GMSCs		12周

表 1

图 1

图 2

图 3

图 4

图 5

图 6

图 7

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干细胞局部应用对缺损面神经再生效果的系统评价

Effect of the local application of stem cells on repairing facial nerve defects: a systematic review

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