白细胞介素-10基因型与墨玉县维吾尔族成人慢性牙周炎易患性的相关性研究

doi:10.7518/hxkq.2017.05.014

摘要/Abstract

摘要：

目的探讨白细胞介素-10（IL-10）基因-597（C/A）位点单核苷酸多态性与新疆维吾尔自治区墨玉县维吾尔族成人慢性牙周炎（CP）之间的相关性。方法随机选取2013年4—5月本课题组对新疆墨玉县维吾尔族成人进行的口腔健康流行病学调查资料及采集的颊黏膜拭子样本共300例,根据纳入排除标准将其分为健康对照组、轻度CP组和中重度CP组,每组各100例。采用四引物等位基因特异性聚合酶链反应扩增法检测受试者IL-10-597位点基因型和等位基因的分布。采用卡方检验、有序多分类Logistic回归等方法进行统计学分析。结果 IL-10-597基因型及等位基因频率在健康对照组、轻度CP、中重度CP组分布的差异均无统计学意义（P>0.05）;抽取样本人群的年龄与CP具有相关性,55~65岁人群患CP的风险是低于35岁人群的25倍（OR=25.56,P<0.001）。结论 IL-10-597（C/A）位点单核苷酸多态性与维吾尔族成人CP的发病风险无明显相关性。

关键词: 白细胞介素-10, 慢性牙周炎, 单核苷酸多态性

Abstract:

Objective This study aims to investigate the association between interleukin (IL)-10-597 (C/A) single-nucleotide polymorphisms and chronic periodontitis of Moyu Uygur population in Xinjiang Uygur Autonomous Region of China. Methods In accordance with the inclusion and exclusion criteria, the buccal swabs of 300 subjects were randomly selected from the epidemiological investigation of Uygur adults in Moyu county on April and May 2013. The study was conducted on a healthy control group, a mild chronic periodontitis group, and a moderate-to-severe chronic periodontitis group, with each comprising 100 samples. The IL-10-597(C/A) site in the promoter sequences was analyzed using the tetra-primer amplification refractory mutation system-polymerase chain reaction method to test the genotype and allele distributions. Statistical analysis was performed using Chi-squared test and ordinal classification Logistic regression analysis. Results The genotype and allelefrequencies of the IL-10-597(C/A) site in the healthy control group, mild chronic periodontitis group, and moderate-to-severe chronic periodontitis group exhibited no significant difference (P>0.05). The age of all the samples was associated with chronic periodontitis. The risk of chronic periodontitis in the people of 55-65 years old was 25 times in the people under the age of 35 (OR=25.56, P<0.001). Conclusion The IL-10-597 (C/A) single-nucleotide polymorphisms in the gene promoter are not associated with chronic periodontitis in Uygur adult population.

Key words: interleukin-10, chronic periodontitis, single nucleotide polymorphisms

中图分类号:

R781.4

张雨慧, 黄萍, 林静, 赵今. 白细胞介素-10基因型与墨玉县维吾尔族成人慢性牙周炎易患性的相关性研究[J]. 华西口腔医学杂志, 2017, 35(5): 514-519.

Yuhui Zhang, Ping Huang, Jing Lin, Jin Zhao. Correlation between interleukin-10 polymorphisms and susceptibility to chronic periodontitis among Uygur adults in the Moyu area[J]. West China Journal of Stomatology, 2017, 35(5): 514-519.

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项目分类		分组
项目分类		健康对照	轻度CP	中重度CP
性别^1）	男	34	50	60
	女	66	50	40
年龄/岁^2）		34.6±11.0	40.3±11.3	53.0±10.0
CAL/mm		NA	1.76±0.55	4.51±1.44
PD/mm^3）		1.26±0.38	2.09±0.50	2.47±0.64

分组	AA/%		AC/%		CC/%		卡方值	P值
分组	观察值	期望值	观察值	期望值	观察值	期望值	卡方值	P值
健康对照	19/19.0	20.0/20.2	52/52.0	50.0/49.5	29/29.0	30.0/30.2	0.820	0.960
轻度CP	25/25.0	23.0/23.3	46/46.5	49.5/50.0	28/28.0	26.0/26.5	0.324	0.850
中重度CP	25/25.0	24.5/24.5	49/49.0	50.0/50.0	26/26.0	25.5/25.5	0.005	0.997

组别	基因型			等位基因n/%
组别	AA	AC	CC	A	C
健康对照	19	52	29	90/45.0	110/55.0
轻度CP	25	46	28	96/48.5	102/51.5
中重度CP	25	49	26	99/49.5	101/50.5
卡方值	1.571			0.893
P值	0.814			0.640

项目分类		OR值	95%CI	P值
性别	男	0.44	0.28~1.41	0.001*
	女	1.00	-	-
年龄/岁	<35	1.00	-	-
	35~	1.80	0.86~3.62	0.101
	45~	7.90	3.93~15.89	<0.001*
	55~65	25.56	12.29~53.14	<0.001*
基因型	AA	0.78	0.41~1.49	0.460
	AC	0.85	0.50~1.47	0.569
	CC	1.00	-	-