牙周非手术治疗对伴二型糖尿病的慢性牙周炎大鼠血清白细胞介素6表达及颈动脉血管壁的影响

doi:10.7518/hxkq.2019.06.004

华西口腔医学杂志 ›› 2019, Vol. 37 ›› Issue (6): 589-593.doi: 10.7518/hxkq.2019.06.004

牙周非手术治疗对伴二型糖尿病的慢性牙周炎大鼠血清白细胞介素6表达及颈动脉血管壁的影响

王苗苗,赵勇,王冲,李豪,石学雪,任秀云()

山西医科大学口腔医学院,太原 030001

收稿日期:2019-03-01 修回日期:2019-07-11 出版日期:2019-12-01 发布日期:2019-11-27
通讯作者: 任秀云 E-mail:rxy611@163.com
作者简介:王苗苗,硕士,E-mail：1522078904@qq.com
基金资助:
国家自然科学基金(81271144)

Influence of periodontal non-surgical therapy on serum interleukin 6 expression and carotid artery wall in rats with periodontitis and type 2 diabetes mellitus

Wang Miaomiao,Zhao Yong,Wang Chong,Li Hao,Shi Xuexue,Ren Xiuyun()

Shanxi Medical University School and Hospital of Stomatology, Taiyuan 030001, China

Received:2019-03-01 Revised:2019-07-11 Online:2019-12-01 Published:2019-11-27
Contact: Xiuyun Ren E-mail:rxy611@163.com
Supported by:
The National Natural Science Foundation of China (81271144)

摘要/Abstract

摘要：

目的探讨牙周非手术治疗对伴二型糖尿病（T2DM）慢性牙周炎（CP）大鼠模型中血清白细胞介素6（IL-6）的表达及颈动脉血管壁的影响。方法将28只6周龄清洁级SD大鼠随机分为A组（正常对照组,7只）、B组（T2DM+CP组,21只）,将B组按照不同干预措施随机分成B1（自然进程组）、B2（机械治疗组）、B3（机械治疗+盐酸米诺环素+抗生素组）,用酶联免疫吸附测定法进行血清IL-6的测定,苏木精-伊红（HE）染色后光镜下观察颈动脉组织的病理变化。结果 A组血管壁形态正常;B1组内皮细胞脱落消失,中膜弹性纤维排列紊乱,平滑肌组织局部坏死呈无定型颗粒,少量钙盐沉积,管壁增厚明显;B2、B3组内皮细胞脱落消失,中膜部分平滑肌细胞空泡变性,血管壁无明显增厚。B1组血清IL-6含量随时间持续升高,高于A组（P<0.001）。各干预组血清IL-6的含量在第一次干预后1周达到峰值,随后逐渐下降,在第二次干预后5周,B2、B3组均显著低于B1组（P<0.001）。结论对于伴有糖尿病的牙周炎大鼠,单纯牙周机械治疗在短期内可能会引起血清IL-6水平升高,远期疗效则可能会降低机体的炎症状态,且伴有颈动脉血管病变的改善;联合使用局部及全身抗生素可能优化其疗效。

关键词: 慢性牙周炎, 二型糖尿病, 白细胞介素6, 颈动脉血管壁, 牙周非手术治疗

Abstract:

Objective To study the effect of periodontal non-surgical treatment on serum interleukin-6 (IL-6) expression and carotid vascular wall in a rat model of chronic periodontitis (CP) with type 2 diabetes mellitus (T2DM).Methods Twenty-eight 6-week-old SD rats were randomly divided into group A (control group, 7 rats) and group B (T2DM+CP group, 21 rats). According to different intervention measures, group B was randomly subdivided into B1 (natural process), B2 (periodontal mechanical treatment), and B3 (periodontal mechanical treatment + 2% minocycline hydrochloride + systemic metronidazole plus amoxicillin). Serum IL-6 was measured by enzyme-linked immunosorbent assay, and carotid tissue was observed under light microscopy after hematoxylin-eosin (HE) staining.Results The carotid artery wall of group A was normal. In group B1, the endothelial cells disappeared, the elastic fibers of the middle membrane were disordered, the local necrosis of smooth muscle tissue was amorphous particles, a small amount of calcium salt was deposited, and the wall thickening was obvious. The endothelial cells in groups B2 and B3 disappeared, and the smooth muscle cells in the middle membrane underwent denaturation. No significant thickening of the vessel wall was noted. The serum IL-6 level in group B1 continually increased with time, and it was higher than that in group A (P<0.001). The IL-6 levels of groups B2 and B3 peaked a week after the first intervention. Subsequently, IL-6 levels gradually decreased. At the last point (five weeks after the second intervention ), the IL-6 levels of groups B2 and B3 were significantly lower than that of group B1 (P<0.001).Conclusion For rats with periodontitis and diabetes, periodontal mechanical treatment may elevate serum IL-6 levels in the short term but might reduce the whole inflammatory state in the long term. Thus, this treatment might be valuable to the improvement of vascular disease. The adjunctive benefits of systemic metronidazole and amoxicillin in non-surgical periodontal therapy are decreased serum IL-6 expression and normal carotid artery.

Key words: chronic periodontitis, type 2 diabetes mellitus, interleukin 6, carotid artery wall, periodontal non-surgical therapy

中图分类号:

R781.4

王苗苗, 赵勇, 王冲, 李豪, 石学雪, 任秀云. 牙周非手术治疗对伴二型糖尿病的慢性牙周炎大鼠血清白细胞介素6表达及颈动脉血管壁的影响[J]. 华西口腔医学杂志, 2019, 37(6): 589-593.

Wang Miaomiao, Zhao Yong, Wang Chong, Li Hao, Shi Xuexue, Ren Xiuyun. Influence of periodontal non-surgical therapy on serum interleukin 6 expression and carotid artery wall in rats with periodontitis and type 2 diabetes mellitus[J]. West China Journal of Stomatology, 2019, 37(6): 589-593.

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组别	第一次干预前1周	第一次干预后1周	第二次干预后1周	第二次干预后3周	第二次干预后5周
A	145.090±41.174	144.428±24.242	153.082±24.026	146.012±4.024	155.856±28.919
B1	153.529±9.492	293.311±17.220^*	318.641±27.406^*	354.849±25.540^*	487.724±18.830^*
B2	206.247±63.609^**##	656.802±54.232^*#	289.060±36.427^*	263.783±28.460^*#	206.890±28.055^**#
B3	225.446±37.231^*##	631.248±37.038^*#	281.432±45.458^*	235.854±21.892^*#	184.652±24.282^#

牙周非手术治疗对伴二型糖尿病的慢性牙周炎大鼠血清白细胞介素6表达及颈动脉血管壁的影响

Influence of periodontal non-surgical therapy on serum interleukin 6 expression and carotid artery wall in rats with periodontitis and type 2 diabetes mellitus

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