分泌型卷曲相关蛋白1及β-连环蛋白在慢性牙周炎患者牙龈组织中的表达

doi:10.7518/hxkq.2018.03.005

华西口腔医学杂志 ›› 2018, Vol. 36 ›› Issue (3): 257-261.doi: 10.7518/hxkq.2018.03.005

分泌型卷曲相关蛋白1及β-连环蛋白在慢性牙周炎患者牙龈组织中的表达

池毓坦(), 许春姣(), 孙晓娟, 李冬梅, 王宏峰, 王苗苗, 贺秀芳

中南大学湘雅医院口腔医学中心,长沙 410008

收稿日期:2017-12-15 修回日期:2018-03-01 出版日期:2018-06-01 发布日期:2018-06-01
作者简介:
池毓坦,硕士,E-mail：337686520@qq.com
基金资助:
教育部留学回国人员科研启动基金[教外司留(2012)940号]

Expression and significance of secreted frizzled-related protein 1 and β-catenin in gingival tissue of patients with chronic periodontitis

Yutan Chi(), Chunjiao Xu(), Xiaojuan Sun, Dongmei Li, Hongfeng Wang, Miaomiao Wang, Xiufang He

Center of Stomatology, Xiangya Hospital of Central South University, Changsha 410008, China

Received:2017-12-15 Revised:2018-03-01 Online:2018-06-01 Published:2018-06-01
Supported by:
The Project-sponsored by Scientific Research Foundation for Returned Overseas Chinese Scholars, State Edu-cation Ministry of China [(2012)940].

摘要/Abstract

摘要：

目的检测分泌型卷曲相关蛋白1（SFRP1）及β-连环蛋白（β-catenin）在慢性牙周炎（CP）患者牙龈组织中的表达及相关性,探讨经典Wnt/β-catenin信号通路在牙周炎发生发展中的作用。方法选取CP患者28例（CP组）,其中中度CP16例,重度CP12例,健康对照者12例（正常组）,收集牙龈组织并记录探诊深度、出血指数及临床附着丧失水平。采用免疫组织化学技术检测SFRP1及β-catenin的表达,双评分法评价阳性染色强度,SPSS 19.0软件进行统计分析。结果正常组SFRP1及β-catenin的着色强度积分值分别为2.16±0.65、1.12±0.51,CP组为3.57±0.45、2.36±0.49,其中中度CP组为3.61±0.40、2.30±0.44,重度CP组为3.52±0.52、2.45±0.55。CP组中SFRP1及β-catenin的表达较正常组升高,差异有统计学意义（P<0.01）。进一步比较,正常组与中度、重度CP组间差异均有统计学意义（P<0.01）,但中度与重度CP组间差异无统计学意义（P>0.05）。SFRP1与β-catenin着色强度的相关系数r=0.657（P<0.01）。两者的表达均与牙周临床指数相关,其中SFRP1与探诊深度的相关性最明显（r=0.723,P<0.01）;β-catenin与出血指数的相关性最强（r=0.697,P<0.01）。结论 SFRP1及β-catenin在CP患者牙龈组织中的表达升高且与牙周破坏相关,两者的异常表达可能促进牙周炎的发生发展。

关键词: 慢性牙周炎, 分泌型卷曲相关蛋白1, β-连环蛋白;

Abstract:

Objective This study aimed to investigate the expression and correlation of secreted frizzled-related protein 1 (SFRP1) and β-catenin in gingival tissues of patients with chronic periodontitis (CP). The role of the classical Wnt/β-catenin signaling pathway in the development of periodontitis was also explored. Methods Twenty-eight patients with CP (CP group) were enrolled in this study. Among them, 16 cases were moderate CP, and 12 demonstrated severe CP. Twelve healthy cases comprised the controls (normal group). Gingival tissue was collected, and the probing depth, bleeding index, and clinical attachment loss were recorded. The expression levels of SFRP1 and β-catenin were detected by immunohistochemistry, and staining intensity was evaluated by double scoring method. SPSS 19.0 was used for statistical analysis. Results The staining strength scores of SFRP1 and β-catenin were 2.16±0.65 and 1.12±0.51 in the normal group, 3.57±0.45 and 2.36±0.49 in the CP group, 3.61±0.40 and 2.30±0.44 in the moderate CP group, and 3.52±0.52 and 2.45±0.55 in the severe CP group, respectively. The expression of SFRP1 and β-catenin in the CP group was higher than that in the normal group (P<0.01). A significant difference was noted between the normal group and the moderate and severe CP groups (P<0.01) but none between the moderate and severe CP groups (P>0.05). A positive correlation was found between the expression of SFRP1 and β-catenin (r=0.657, P<0.01). The expression levels of β-catenin and SFRP1 were related to periodontal indexes. The correlation between the expression of SFRP1 and probing depth was most significant (r=0.723, P<0.01), as well as that between β-catenin and bleeding index (r=0.697, P<0.01). Conclusion Patients with CP exhibit elevated expression of SFRP1 and β-catenin in gingival tissues, and this event is related to the degree of periodontal destruction. Abnormal expression of SFRP1 and β-catenin may promote the development of periodontitis.

Key words: chronic periodontitis, secreted frizzled-related protein 1, β-catenin;

中图分类号:

R781.4

池毓坦, 许春姣, 孙晓娟, 李冬梅, 王宏峰, 王苗苗, 贺秀芳. 分泌型卷曲相关蛋白1及β-连环蛋白在慢性牙周炎患者牙龈组织中的表达[J]. 华西口腔医学杂志, 2018, 36(3): 257-261.

Yutan Chi, Chunjiao Xu, Xiaojuan Sun, Dongmei Li, Hongfeng Wang, Miaomiao Wang, Xiufang He. Expression and significance of secreted frizzled-related protein 1 and β-catenin in gingival tissue of patients with chronic periodontitis[J]. West China Journal of Stomatology, 2018, 36(3): 257-261.

图/表 7

表 1

图 1

图 2

表 2

表 3

表 4

表 5

参考文献 13

[1]	Duan P, Bonewald LF.The role of the Wnt/β-catenin sig-naling pathway in formation and maintenance of bone and teeth[J]. Int J Biochem Cell Biol, 2016, 77(Pt A): 23-29.
[2]	Maekawa T, Kulwattanaporn P, Hosur K, et al.Differential expression and roles of secreted frizzled-related protein 5 and the wingless homolog Wnt5a in periodontitis[J]. J Dent Res, 2017, 96(5): 571-577.
[3]	Napimoga MH, Nametala C, da Silva FL, et al. Involvement of the Wnt-β-catenin signalling antagonists, sclerostin and dickkopf-related protein 1, in chronic periodontitis[J]. J Clin Periodontol, 2014, 41(6): 550-557.
[4]	Esteve P, Bovolenta P.The advantages and disadvantages of sfrp1 and sfrp2 expression in pathological events[J]. Tohoku J Exp Med, 2010, 221(1): 11-17.
[5]	袁海波, 金晶, 许春姣, 等. 分泌型卷曲相关蛋白1与慢性牙周炎的相关分析[J]. 华西口腔医学杂志, 2013, 31(6): 615-618.
	Yuan HB, Jin J, Xu CJ, et al.Exploring the relationship be-tween secreted frizzled-related protein 1 and chronic perio-dontitis[J]. West Chin J Stomatol, 2013, 31(6): 615-618.
[6]	Boudin E, Fijalkowski I, Piters E, et al.The role of extra-cellular modulators of canonical Wnt signaling in bone me-tabolism and diseases[J]. Semin Arthritis Rheum, 2013, 43(2): 220-240.
[7]	Wang FS, Lin CL, Chen YJ, et al.Secreted frizzled-related protein 1 modulates glucocorticoid attenuation of osteogenic activities and bone mass[J]. Endocrinology, 2005, 146(5): 2415-2423.
[8]	Matzelle MM, Gallant MA, Condon KW, et al.Resolution of inflammation induces osteoblast function and regulates the Wnt signaling pathway[J]. Arthritis Rheum, 2012, 64(5): 1540-1550.
[9]	Li CH, Amar S.Inhibition of SFRP1 reduces severity of periodontitis[J]. J Dent Res, 2007, 86(9): 873-877.
[10]	Jang J, Ha JH, Chung SI, et al.Beta-catenin regulates NF-kappaB activity and inflammatory cytokine expression in bronchial epithelial cells treated with lipopolysaccharide[J]. Int J Mol Med, 2014, 34(2): 632-638.
[11]	Tickenbrock L, Schwable J, Strey A, et al.Wnt signaling regulates transendothelial migration of monocytes[J]. J Leu-koc Biol, 2006, 79(6): 1306-1313.
[12]	Ma B, Hottiger MO.Crosstalk between Wnt/beta-Catenin and NF-kappaB signaling pathway during inflammation[J]. Front Immunol, 2016(7): 378.
[13]	Guo H, Nagy T, Pierce M.Post-translational glycoprotein modifications regulate colon cancer stem cells and colon adenoma progression in Apc(min/+) mice through altered Wnt receptor signaling[J]. J Biol Chem, 2014, 289(45): 31534-31549.

项目	CP组（n=28）	正常组（n=12）	P值
年龄/岁	38.11±8.29	32.40±3.98	<0.05
性别（男/女）	15/13	7/5	>0.05
PD/mm	4.09±1.66	1.73±0.22	<0.05
BI	3.30±0.71	0.25±0.20	<0.05
CAL/mm	4.08±1.66	0.12±0.37	<0.05

组别	积分值	P值（两两比较）			方差分析
组别	积分值	正常	中度CP	重度CP	F值	P值
正常	2.16±0.65	-	<0.01*	<0.01**	28.36	<0.01
中度CP	3.61±0.40	<0.01*	-	0.875^Δ
重度CP	3.52±0.52	<0.01**	0.875^Δ	-

组别	积分值	P值（两两比较）			方差分析
组别	积分值	正常	中度CP	重度CP	F值	P值
正常	1.12±0.51	-	<0.01*	<0.01**	23.57	<0.01
中度CP	2.30±0.44	<0.01*	-	0.71^Δ
重度CP	2.45±0.55	<0.01**	0.71^Δ	-

牙周临床指标	r值		P值
牙周临床指标	SFRP1	β-catenin	P值
PD	0.723	0.620	<0.01
CAL	0.631	0.610	<0.01
BI	0.715	0.697	<0.01

[1]	姜健红, 石兴莲, 何权敏, 高丽, 杨琨, 王太萍, 李哲臻, 刘梅. 老年慢性牙周炎患者健康素养与生活质量的相关性研究[J]. 华西口腔医学杂志, 2023, 41(6): 694-700.
[2]	张晨, 候珍珍, 宗颖睿. 慢性牙周炎与慢性阻塞性肺疾病的潜在共同分子机制及其转录因子初探[J]. 华西口腔医学杂志, 2023, 41(5): 533-540.
[3]	戴振宁, 郑蔚晗, 利时雨. 核因子κB受体活化因子配体和肿瘤坏死因子α经炎性牙周膜干细胞外泌体促进破骨细胞分化[J]. 华西口腔医学杂志, 2022, 40(4): 377-385.
[4]	张杨珩, 李厚轩, 闫福华, 谭葆春. 牙周内窥镜辅助龈下刮治对残留牙周袋疗效的随机对照临床研究[J]. 华西口腔医学杂志, 2020, 38(5): 532-536.
[5]	常雅琴, 房方方, 秦莎莎, 董迎春, 陈斌. 中重度慢性牙周炎患者牙周洁刮治前后血清C反应蛋白水平变化的系统评价和Meta分析[J]. 华西口腔医学杂志, 2020, 38(4): 385-392.
[6]	柏银, 白远亮, 赖静, 黄姣. 克拉霉素辅助治疗慢性牙周炎疗效的Meta分析[J]. 华西口腔医学杂志, 2020, 38(3): 290-296.
[7]	王苗苗, 赵勇, 王冲, 李豪, 石学雪, 任秀云. 牙周非手术治疗对伴二型糖尿病的慢性牙周炎大鼠血清白细胞介素6表达及颈动脉血管壁的影响[J]. 华西口腔医学杂志, 2019, 37(6): 589-593.
[8]	王旭,石磊,应绚,童钰鑫,张佳喻,石旭妍,陈悦. 吸烟对慢性牙周炎牙龈微循环影响的初步研究[J]. 华西口腔医学杂志, 2019, 37(5): 485-489.
[9]	孙晋,刘芸,屈茜,曲娟,罗纬,张锋,吴敏. 组蛋白乙酰化在慢性牙周炎来源牙周膜干细胞成骨分化中的作用[J]. 华西口腔医学杂志, 2019, 37(1): 102-105.
[10]	李小英,李倩,崔博淼,王利伟,樊亚平,陈娇,冯云,张平. 慢性牙周炎患者唾液诱导巨噬细胞分化和活化的研究[J]. 华西口腔医学杂志, 2018, 36(5): 508-513.
[11]	白远亮, 郑苔菁, 张芷玮, 甘祎, 黄姣. 盐酸米诺环素联合翻瓣术辅助治疗慢性牙周炎疗效的Meta分析[J]. 华西口腔医学杂志, 2018, 36(4): 421-427.
[12]	殷艳丽, 许春梅, 吴亚菲, 赵蕾. 龈下刮治和根面平整术联合Nd: YAG激光治疗慢性牙周炎的疗效评价[J]. 华西口腔医学杂志, 2017, 35(6): 618-624.
[13]	张雨慧, 黄萍, 林静, 赵今. 白细胞介素-10基因型与墨玉县维吾尔族成人慢性牙周炎易患性的相关性研究[J]. 华西口腔医学杂志, 2017, 35(5): 514-519.
[14]	杨镰徽, 连梅菲, 胡钟月, 冯云枝. 慢性牙周炎与高脂血症相关性的Meta分析[J]. 华西口腔医学杂志, 2017, 35(4): 419-426.
[15]	李晓光, 王一珠, 郭斌. 慢性牙周炎中肿瘤坏死因子ɑ对骨髓间充质干细胞成骨分化的调控作用[J]. 华西口腔医学杂志, 2017, 35(3): 334-338.

分泌型卷曲相关蛋白1及β-连环蛋白在慢性牙周炎患者牙龈组织中的表达

Expression and significance of secreted frizzled-related protein 1 and β-catenin in gingival tissue of patients with chronic periodontitis

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 13

相关文章 15

编辑推荐

Metrics

本文评价