K (lysine) acetyltransferase 2A affects the osteogenic differentiation of periodontal ligament stem cells through the canonical Wnt pathway

doi:10.7518/hxkq.2018.01.008

Abstract

Abstract:

Objective This study aims to investigate the mechanism of K (lysine) acetyltransferase 2A (KAT2A) regulation and control on the osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Methods The expression levels of KAT2A in PDLSCs were compared from each generation of the normal (H-PDLSCs) and periodontitis tissues (P-PDLSCs). The influences of KAT2A gene interference on the osteogenic differentiation of PDLSCs were also detected. In addition, the influences of the KAT2A gene interference to the canonical Wnt pathway and ligands were detected. The upstream and down-stream relationships between KAT2A and canonical Wnt pathway were also determined. Results The decreased expression of KAT2A in PDLSCs from the inflammatory tissue in each generation was compared with that in PDLSCs from the healthy tissue, and the difference was statistically significant (P<0.05). When the KAT2A gene was disrupted, the osteogenesis ability of PDLSC was declined, and the difference was statistically significant (P<0.05). The canonical Wnt pathway was activated, and the antagonist Dickkopf-1 (DKK-1) was reduced. After the DKK-1 addition, the osteogenic differentiation of the disturbed PDLSCs was recovered, and KAT2A was unaffected. Conclusion The KAT2A expression in PDLSCs was decreased because of perio-dontitis. The classical Wnt pathway was activated to inhibit the osteogenic differentiation of the cells.

Key words: periodontal ligament stem cells, perio-dontitis, acetyltransferase, osteogenic differentiation, classical Wnt pathway

CLC Number:

R781

Wucheng Guo, Jieli Cheng, Zhengyi Yang, Yi Zhang, Enliang He, Jun Qian, Jingjing Song, Jin Sun, Lin Yuan. K (lysine) acetyltransferase 2A affects the osteogenic differentiation of periodontal ligament stem cells through the canonical Wnt pathway[J]. West China Journal of Stomatology, 2018, 36(1): 39-45.

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基因名称	引物序列（5’—3’）
GAPDH	F：CTGCAAGAACAGCATTGCAT
	R：GACCACCTGGTCCTCAGTGT
KAT2A	F：CCAAATCAAGTCTCACCCCAGT
	R：GGAAGCGGATGACCTCGTAG
WNT1	F：CGGGCAACAACCAAAGTCG
	R：CGTTCACAATACCCCACCAT
WNT2	F：ACAGCAGGCCGTGTGTGTGCAA
	R：AGGCAGTCCTGACAGCGCAC
WNT2B	F：GACCGGGACCACACCGTCTTTGG
	R：GGTGGAGGGTGGAGGAAGGTG
WNT3A	F：CTCGGATACTTCTTACTCCTCTGC
	R：CCTGGATGCCAATCTTGATG
WNT8A	F：GATGCCAGAGCCCTGATGAA
	R：GCCTGGTCATACTTGGCCTT
WNT10A	F：TTCTTCCTACTGCTGCTGGC
	R：TTAGGCACACTGTGTTGGCA
WNT10B	F：CAACACCGTGTGCTTGACG
	R：CAGCCAGCATGGAGAAGGAA
DKK-1	F：GGGAATTACTGCAAAAATGGAATA
	R：ATGACCGGAGACAAACCAGAAC
骨硬化蛋白	F：AGCTGGAGAACAACAAGACCA
	R：CATCGGTCACGTAGCGGG
Sfrp1	F：GATGCAGGAGGCTCAGGTGAT
	R：GCTGGCAACAGGTCAGAACG

干细胞标记物	H-PDLSCs	P-PDLSCs
Ctr-PE（PE通道对照组）	0.11	0.13
Ctr-FITC（FITC通道对照组）	0.08	0.05
STRO-1	31.24	37.61
CD146	74.92	77.17
CD105	80.52	78.34
CD90	98.35	99.08
CD34	0.31	0.26
CD14	0.21	0.14

细胞传代数	H-PDLSCs	P-PDLSCs	P值
P0	1.00±0.15	0.62±0.13	<0.01
P2	1.00±0.18	0.62±0.05	<0.01
P4	1.00±0.11	0.68±0.06	<0.05
P6	1.00±0.09	0.71±0.07	<0.05