West China Journal of Stomatology ›› 2025, Vol. 43 ›› Issue (4): 547-558.doi: 10.7518/hxkq.2025.2024409

• Basic Research • Previous Articles     Next Articles

miR-302a-3p targeting lysosomal-associated membrane protein 5 inhibits the invasion and metastasis of oral squamous cell carcinoma

Yu Li1,2(), Zhou Tiejun3, Wu Xiao2, Lin Xinhong2, Zhang Xiaoyan1,2, Lai Yongxian2,4, Liao Xinyue2,5, Si Hang2,5, Feng Yun2,5, Jian Jie6(), Feng Yan2,5()   

  1. 1.Dept. of Periodontal and Oral Mucosal Disease, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, China
    2.Oral & Maxillofacial Reconstruction and Regeneration of Luzhou Key Laboratory, Luzhou 646000, China
    3.Dept. of Pathology, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China
    4.Dept. of Preventive Health Care, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, China
    5.Dept. of Pediatric Dentistry, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, China
    6.Deyang Stomatological Hospital, Deyang 618000, China
  • Received:2024-11-08 Revised:2025-01-23 Online:2025-08-01 Published:2025-08-29
  • Contact: Jian Jie,Feng Yan E-mail:yulixt126@swmu.edu.cn;jianjie_4950@163.com;fyxt126@swmu.edu.cn
  • Supported by:
    National Natural Science Foundation of China(82403404);Sichuan Science and Technology Program(2024JDRC0040);Youth Innovation Project of Sichuan Provincial Medical Association(Q23028);Luzhou Science and Technology Program(2023JYJ002);Deyang Stomatological Hospital-Southwest Medical University Science and Technology Strategic Cooperation Project(2024DYKQXNYD03);2024 National and School Undergraduate Innovation and Entrepreneurship Program(202410632009)

Abstract:

Objective This study aimed to explore the expression of lysosomal-associated membrane protein 5 (LAMP5) and microRNA (miR)-302a-3p in oral squamous cell carcinoma (OSCC) and their functional mechanism on the invasion and metastasis of OSCC. Methods The expression of LAMP5 in OSCC and its sensitivity as a prognostic indicator were analyzed on the basis of The Cancer Genome Atlas database. Western blot, quantitative reverse transcription polymerase chain reaction, and cell immunocytochemistry were used to detect the expression of LAMP5 in OSCC tissues and cells. The effect of LAMP5 on the proliferation, migration, and invasion of OSCC cells was evaluated through cell counting kit-8, immunocytochemistry, migration, and invasion assays, respectively. The miRNA targeting prediction websites were used to predict the miR that regulates LAMP5 and verify the targeted regulatory effect of miR-302a-3p on LAMP5. The effect of LAMP5 knockdown on OSCC tumor growth was evaluated in a nude mouse tumorigenesis model. Results LAMP5 was highly expressed in OSCC tissues and cells. It showed high sensitivity in the early diagnosis of OSCC. LAMP5 knockdown significantly inhibited the proliferation, migration, and invasion of OSCC cells, whereas LAMP5 overexpression increased these cell activities. The expression of LAMP5 was regulated by miR-302a-3p. In vivo, LAMP5 knockdown significantly inhibited the growth of OSCC tumor. Conclusion LAMP5 promotes the malignant progression of OSCC by enhancing the proliferation, migration, and invasion of OSCC cells. The expression of LAMP5 is negatively regulated by miR-302a-3p.

Key words: microRNA-302a-3p, lysosomal-associated membrane protein 5, oral squamous cell carcinoma, inva-sion and metastasis

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