West China Journal of Stomatology

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Expression of full-length spleen tyrosine kinase in the oral squamous cell carcinoma and its relationship with tumor invasion and metastasis

Wang Zhao1,2, Chen Jie1,2, Chu Weiming1,2, Da Mingjie1,2, Ma Lu1,2, Wu Min3, Zhong Yi3, Wang Zilu1, Song Xiaomeng1,2, Wu Yunong1,2.   

  1. 1. Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing 210029, China; 2. Dept. of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, China; 3. Dept. of Pathology, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, China
  • Received:2015-03-12 Revised:2015-07-05 Online:2015-10-01 Published:2015-10-01

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Abstract:

Objective  To investigate the expression of full-length spleen tyrosine kinase [SYK (L)] mRNA and protein in human oral squamous cell carcinoma (OSCC) as well as its possible effects on the invasion and metastasis of OSCC. Methods  The expression of SYK (L) was detected in 27 cases of OSCC tissues and its matched adjacent non-cancerous tissues by real-time quantitative polymerase chain reaction (RT-qPCR), Western blot, and immunohistochemistry. Fourteen cases of normal oral gingival tissues were also analyzed as a normal control. Results  Reduced mRNA and protein expression of SYK (L) in OSCC tissues was observed compared with that in normal oral gingival tissues (P<0.01) and adjacent non-cancerous tissues (P<0.05). SYK(L) expression was significantly associated with lymph-node metastasis (P<0.05). Conclusion  SYK(L) is a candidate tumor suppressor for OSCC tissues, and has an inhibitive effect on the initiation, proliferation, and lymph-node metastasis of human OSCC.

Key words: oral squamous cell carcinoma, full-length spleen tyrosine kinase, lymph node metastasis, alternative-splice

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