Abstract:

Objective To explore the expression of X-linked inhibitor of apoptosis protein（XIAP） in Tca8113 cell, and to investigate its relationship to the chemoresistance. Methods The Tca8113 cell line was cultured by IMDM and the concentration of Pingyangmycin（PYM） added to Tca8113 cell line was increased gradually and continually, which was to induce the PYM-resistance in Tca8113 cell line. The sensitivity of Tca8113 cell to PYM and expression of XIAP were measured with methyl thiazolyl tetrazolium（MTT） chromatometry and reverse transcriptionpolymerase chain raction（RT-PCR）. The XIAP level in the cells and its chemoresistance to PYM were analyzed by linear regression. Results The IC50 of Tca8113-1-10 group and Tca8113-10-10 group were（12.758±0.030）, （18.986± 0.150）μg·mL-1 respectively. The IC50 of Tca8113-1-20 group and Tca8113-10-20 group increased to（26.302±0.072）, （35.294±0.115）μg·mL-1 respectively. There was a relation between XIAP and the drug-resistance in Tca8113 cell. Conclusion XIAP may play an important role in the chemoresistance which might serve as a new therapeutic target for oral squamous cell carcinoma.

Key words: oral squamous cell carcinoma, X-linked inhibitor of apoptosis protein, chemoresistance