关键词: 无翅型MMTV整合位点家族成员5A, 受体酪氨酸激酶样孤儿受体2, 牙囊, 破骨细胞

Abstract:

Objective  To observe the expression of wingless-type MMTV integration site family, member 5A (Wnt5A)/ receptor tyrosine kinase-like orphan receptor 2 (Ror2) signal in the dental follicle cells during the normal eruption of the teeth as well as to explore the relationship between the expression of dental follicle cells and the formation of mature osteoclasts and eruption of the teeth. Methods   The mandibulars of 1–13 d old SD rats were separated to observe the growth and development of the teeth and alveolar bone through hematoxylin-eosin(HE) staining. Ror2 and Wnt5A expressions in rat dental follicle were also observed through immunohistochemistry. Dental follicle cells from the lower first intact molar germs of 5-6-day old SD rats were separated and cultured. Results  On the second day after birth, the dental follicle began to differentiate into periodontal tissues, but no obvious changes were observed in the alveolar bone one to three days after birth. On the fourth day, the number of osteoclasts increased significantly. The results of immunohistochemistry showed that Wnt5A was not significantly expressed in rat dental follicle tissues before the fourth day, but positive expression was expressed in the next day and continued to express to thirteenth days. Ror2 was expressed in the rat dental follicle at postnatal days 1–3, but weak expression was found in days 4–13. Conclusion  Wnt5A and Ror2 expressions in the process of tooth eruption have specific time distributions, suggesting that these expressions may participate in the regulation of the eruption of the teeth.

Key words: wingless-type MMTV integration site family, member 5A, receptor tyrosine kinase-like orphan receptor 2, dental follicle, osteoclast