神经营养素3促进人牙囊细胞成骨分化

doi:10.7518/hxkq.2018.01.005

华西口腔医学杂志 ›› 2018, Vol. 36 ›› Issue (1): 23-28.doi: 10.7518/hxkq.2018.01.005

神经营养素3促进人牙囊细胞成骨分化

雷维^1,²(), 马玥^1,², 任嫒姝^1,³()

1.重庆医科大学附属口腔医院正畸科
2.口腔疾病与生物医学重庆市重点实验室
3.重庆市高校市级口腔生物医学工程重点实验室,重庆 401147

收稿日期:2017-06-10 修回日期:2017-10-12 出版日期:2018-02-07 发布日期:2018-02-01
作者简介:
雷维,硕士,E-mail:1091979798@qq.com
基金资助:
[基金项目] 中国博士后科学基金（2013M542248）;2016年重庆高校创新团队建设计划（CXTDG201602006）;重庆市高校市级口腔生物医学工程重点实验室资助项目[渝教科(2014)55号]

Neurotrophin 3 promotes osteogenic differentiation of human dental follicle cells

Wei Lei^1,²(), Yue Ma^1,², Aishu Ren^1,³()

1. Dept. of Orthodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China
2. Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China
3. Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China

Received:2017-06-10 Revised:2017-10-12 Online:2018-02-07 Published:2018-02-01
Supported by:
Supported by: China Postdoctoral Science Foundation (2013M542248);Program for Innovation Team Building at Institutions of Higher Education in Chongqing in 2016 (CXTDG201602006);Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education [(2014)55].

摘要/Abstract

摘要：

目的研究神经营养素3（NT-3）对人牙囊细胞（hDFCs）成骨分化的影响。方法体外分离并培养hDFCs,免疫细胞化学染色法鉴定细胞来源,CCK-8法检测不同质量浓度NT-3对hDFCs增殖活性的影响,同时检测NT-3对hDFCs的碱性磷酸酶（ALP）活性,以及骨形态发生蛋白-2（BMP-2）、骨钙素（OCN）mRNA相对表达量的影响,并用茜素红染色法检测矿化情况。结果波形丝蛋白和角蛋白染色结果表明hDFCs为间充质细胞来源。NT-3对hDFCs增殖活性无明显影响;当NT-3质量浓度为25、50、100 ng·mL^-1时能明显增强ALP活性,提高BMP-2、OCN mRNA的相对表达量,与对照组相比差异有统计学意义（P<0.05）,NT-3质量浓度为100 ng·mL^-1时BMP-2、OCN mRNA的相对表达量达到最高。当NT-3质量浓度为50、100 ng·mL^-1时矿化结节数量最多。结论适宜质量浓度的NT-3能促进hDFCs的成骨分化。

关键词: 神经营养素3, 人牙囊细胞, 成骨分化

Abstract:

Objective This study aims to investigate the effect of neurotrophin 3 (NT-3) on the osteogenic differentiation of human dental follicle cells (hDFCs). Methods hDFCs were isolated and cultured in vitro. Immunocytochemical staining was used to identify the origin of hDFCs. The effects of different NT-3 concentrations on hDFCs proliferation were detected by using CCK-8 assay. The alkaline phosphatase (ALP) activities and mRNA expression levels of bone morphogenetic protein-2 (BMP-2) and osteocalcin (OCN) were determined to investigate the effects of NT-3 on hDFCs osteogenesis. The difference in the number of mineralized nodules was detected using alizarin red staining. Results Vimentin and cytokeratin staining results showed that hDFCs originated from the mesenchymal cells. NT-3 exerted no evident effect on hDFCs proliferation. The ALP activity and the BMP-2 and OCN mRNA expression levels of hDFCs were signiﬁcantly improved under treatment with different NT-3 concentrations (25, 50, and 100 ng·mL^-1) compared with those in the control group. BMP-2 and OCN mRNA relative expression levels of hDFCs reached the highest when the NT-3 concentration was 100 ng·mL^-1. The number of mine-ralized nodules reached the maximum when the hDFCs were treated with 50 and 100 ng·mL^-1 NT-3. Conclusion Appropriate mass concentration of NT-3 can promote the osteogenic differentiation of hDFCs.

Key words: neurotrophin 3, human dental follicle cells, osteogenic differentiation

中图分类号:

R780.2

雷维, 马玥, 任嫒姝. 神经营养素3促进人牙囊细胞成骨分化[J]. 华西口腔医学杂志, 2018, 36(1): 23-28.

Wei Lei, Yue Ma, Aishu Ren. Neurotrophin 3 promotes osteogenic differentiation of human dental follicle cells[J]. West China Journal of Stomatology, 2018, 36(1): 23-28.

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神经营养素3促进人牙囊细胞成骨分化

Neurotrophin 3 promotes osteogenic differentiation of human dental follicle cells

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