关键词: 代谢组学, 核磁共振技术, 细菌, 鉴定

Abstract:

Objective To evaluate the feasibility of employing metabonomics method in identification of oral pathogenic bacteria. Methods The Streptococcus mutans ATCC25175 and Actinomyces viscosus ATCC15987 were respectively inoculated in same certain culture medium. The growth curves of the inoculated bacteria were drown by turbidimetry. The culture solutions in four different growth phases of the both bacteria were used to test with the 1H- Nuclear magnetic resonance（1H-NMR） spectroscopy respectively. The data of 1H-NMR spectroscopy results were analyzed by principal components analysis（PCA）. Results The PCA showed the obvious clustering phenomena and the points of two group data stayed differentially together by two clusters. Therefore, the NMR - based metabonomics profiles can discriminate the two different kind of bacteria. Conclusion The metabonomics can be expected to be a kind of promising useful method in quick discrimination of oral pathogenic bacteria.

Key words: metabonomics, nuclear magnetic resonance, bacterium, identification