sortase A与变异链球菌致龋性关系的代谢组学研究

doi:10.7518/hxkq.2018.04.003

华西口腔医学杂志 ›› 2018, Vol. 36 ›› Issue (4): 360-366.doi: 10.7518/hxkq.2018.04.003

sortase A与变异链球菌致龋性关系的代谢组学研究

宋颖^1,²(), 周京琳³, 何远丽¹, 李伟¹, 邹玲⁴()

1.口腔疾病研究国家重点实验室国家口腔疾病临床医学研究中心四川大学华西口腔医院,成都 610041
2.重庆医科大学附属口腔医院牙体牙髓科,口腔疾病与生物医学重庆市重点实验室,重庆 401147
3.口腔疾病研究国家重点实验室国家口腔疾病临床医学研究中心四川大学华西口腔医院口腔颌面外科
4.牙体牙髓病科,成都 610041

收稿日期:2017-12-11 修回日期:2018-04-09 出版日期:2018-08-01 发布日期:2018-08-01
作者简介:
宋颖,硕士,E-mail：manbudaokou@163.com
基金资助:
国家自然科学基金（81570974）;四川省科技厅基金（20-15JY0260）

Link between sortase A function and cariogenicity of Streptococcus mutans: a preliminary metabolomics analysis

Ying Song^1,²(), Jinglin Zhou³, Yuanli He¹, Wei Li¹, Ling Zou⁴()

1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
2. Dept. of Conservative Dentistry and Endodontics, Stomatological Hospital of Chongqing Medical University, Chongqing Key Labo-ratory of Oral Diseases and Biomedical Sciences,Chongqing 401147, China
3. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
4. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Conservative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China

Received:2017-12-11 Revised:2018-04-09 Online:2018-08-01 Published:2018-08-01
Supported by:
The National Natural Science Foundation of China (81570974).;The Project of Science and Technology Depart-ment of Sichuan Province (2015JY0260).

摘要/Abstract

摘要：

目的探索srtA基因编码的转肽酶sortase A（SrtA）影响变异链球菌致龋性的机制。方法采用基于¹H核磁共振波谱（NMR）的代谢组学方法,比较野生型变异链球菌UA159与srtA基因缺陷型变异链球菌UA159的胞外代谢产物;联合使用主成分分析和正交偏最小二乘法—判别分析鉴定不同菌株之间代谢产物的差异。结果大多数有差异性的代谢产物是未知的,可识别的差异性代谢产物有亮氨酸和缬氨酸（δ 0.92×10^-6~1.20×10^-6）、乳酸（δ 1.28×10^-6）、酮戊二酸（δ 3.00×10^-6）、甘氨酸（δ 3.60×10^-6）。结论在进一步完善微生物代谢产物数据库的基础上,¹H NMR代谢组学可用于龋病病因的研究。

关键词: 代谢组学, 变异链球菌, sortase A, ¹H核磁共振波谱, 主成分分析, 正交偏最小二乘法—判别分析;

Abstract:

Objective This study intends to explore the mechanism underlying the support of sortase A (SrtA) of the cario-genicity of Streptococcus mutans (S. mutans). Methods We performed a metabonomics study based on ¹H nuclear magnetic resonance spectroscopy (NMR), in which we compared the extracellular metabolites of wild-type S. mutans UA159 with those of its SrtA-deficient strain. Metabolite differences among strains were identified using a combination of principal component analysis and orthogonality partial least square discriminant analysis. Results Several differences corresponding mostly to unknown metabolites were identified. Some amino acids such as leucine and valine (δ 0.92×10^-6-1.20×10^-6), lactic acid ( δ1.28×10^-6), oxoglutaric acid (δ 3.00×10^-6), and glycine (δ 3.60×10^-6) differed among strains. Conclusion This work establishes the feasibility of using ¹H NMR-based metabonomics to provide leads for research into molecular factors that promote caries. The database of microbial metabolites should be also improved in further studies.

Key words: metabonomics, Streptococcus mutans, sortase A, ¹H nuclear magnetic resonance spectroscopy, princi-pal component analysis, orthogonality partial least squares discrimination analysis

中图分类号:

R780.2

宋颖, 周京琳, 何远丽, 李伟, 邹玲. sortase A与变异链球菌致龋性关系的代谢组学研究[J]. 华西口腔医学杂志, 2018, 36(4): 360-366.

Ying Song, Jinglin Zhou, Yuanli He, Wei Li, Ling Zou. Link between sortase A function and cariogenicity of Streptococcus mutans: a preliminary metabolomics analysis[J]. West China Journal of Stomatology, 2018, 36(4): 360-366.

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代谢产物	变量/×10^-6	VIP
亮氨酸和缬氨酸	0.96	1.534 33
	0.92	1.319 41
	1.08	1.306 00
乳酸	1.28	1.236 70
甘氨酸	3.60	1.078 84
酮戊二酸	3.00	1.347 58

sortase A与变异链球菌致龋性关系的代谢组学研究

Link between sortase A function and cariogenicity of Streptococcus mutans: a preliminary metabolomics analysis

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