关键词: 代谢组学, 腭裂, 维生素B12

Abstract:

Objective Metabonomic analysis has been increasingly used to monitor metabolic abnormalities in cells and their micro-environment in order to detect the biomarkers recently. This study evaluated the feasibility of applying 1H-nuclear magnetic resonance（1H-NMR） based metabonomic method to detect the differences of the early development of cleft palate in the plasma from control group and experimental group. Methods Pregnant mice（inbred C57BL/6J strain） with vitamin B12 injected only were assigned as the control group, pregnant mice with excessive Dex, injected after vitamin B12 as the experimental group, each group includes 12 mice. And the effect of B12 to rate of cleft palate was observed. The technology of nuclear magnetic resonance（NMR） was used to detect the endogenous small molecule metabolites. Finally, changes of metabolites ingredients were ascertained by using the method of principal component analysis（PCA）. Results There was significant difference in PCA scores plot between the two groups according to whether cleft palate occured. Conclusion The 1H-NMR based metabonomic approach might be used as a feasible and efficient method for a deep exploration of the pathogenesis of cleft lip and palate and an early exploration of the mechanism of vitamin B12.

Key words: metabonomics, cleft palate, vitamin B12