关键词: 转化生长因子-&beta, 1, 白细胞介素-10, 单核苷酸多态性, 复发性口腔溃疡

Abstract:

Objective  To explore the possible relationship between recurrent aphthous ulcer (RAU) and single nucleotide polymorphism (SNP) of transforming growth factor-β1 (TGF-β1)-509T/C and interleukin-10 (IL-10)-1082A/G sites. Methods  A total of 138 RAU patients were recruited for this study. The control group consisted of 124 subjects. TGF-β1-509T/C and IL-10-1082A/G sites were detected by restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) and sequence specific primer-polymerase chain reaction (SSP-PCR). Relative risk ratios were estimated by odds ratios (OR) and 95% confidence interval (95%CI). Results  Significant differences were found in the genotype frequencies or allele frequencies of TGF-β1-509T/C and IL-10-1082A/G sites between the RAU patients and controls (P<0.05). CT genotype (OR=1.231, 95%CI=0.702–2.160), TT genotype (OR=2.482, 95%CI=1.250–4.927), and T allele (OR=1.465, 95%CI=1.036–2.074) at the TGF-β1-509 site exhibited high risks. AG genotype (OR=1.391, 95%CI=0.808–2.396), GG genotype (OR=4.165, 95%CI=1.944–8.924), and G allele (OR=2.134, 95%CI=1.474–3.089) at the IL-10-1082A/G site also showed high risks. Conclusion  TGF-β1-509T/C and IL-10-1082A/G sites are associated with the risk of RAU. The TGF-β1 gene-509T allele and IL-10 gene-1082G allele may serve as genetic determinants for RAU.

Key words: transforming growth factor-β1, interleukin-10, single nucleotide polymorphism, recurrent aphthous ulcer