牙龈卟啉单胞菌来源的脂多糖通过X盒结合蛋白1调控脂肪细胞胰岛素信号通路的机制研究

doi:10.7518/hxkq.2022.02.004

华西口腔医学杂志 ›› 2022, Vol. 40 ›› Issue (2): 148-154.doi: 10.7518/hxkq.2022.02.004

牙龈卟啉单胞菌来源的脂多糖通过X盒结合蛋白1调控脂肪细胞胰岛素信号通路的机制研究

陆佳艺¹(), 伍倩琪¹, 陈伊燕¹, 叶蕾蕾², 苏媛¹^,²()

^1.南方医科大学顺德医院（佛山市顺德区第一人民医院）口腔中心，顺德 528300
^2.南方医科大学口腔医院牙周科，广州 510280

收稿日期:2021-03-08 修回日期:2021-07-25 出版日期:2022-04-01 发布日期:2022-04-01
通讯作者: 苏媛 E-mail:470866002@qq.com;maggie_suyuan@126.com
作者简介:陆佳艺，硕士，E-mail：470866002@qq.com
基金资助:
国家自然科学基金(81400509)

Mechanism of Porphyromonas gingivalis-lipopolysaccharide in regulating the insulin signaling pathway in adipocytes via X-box binding protein 1

Lu Jiayi¹(), Wu Qianqi¹, Chen Yiyan¹, Ye Leilei², Su Yuan¹^,²()

^1.Stomatology Center, Shunde Hospital, Southern Medical University (The First People, s Hospital of Shunde), Shunde 528300, China
^2.Dept. of Periodontology, Stomatological Hospital, Southern Medical University, Guangzhou 510280, China

Received:2021-03-08 Revised:2021-07-25 Online:2022-04-01 Published:2022-04-01
Contact: Su Yuan E-mail:470866002@qq.com;maggie_suyuan@126.com
Supported by:
Young Scientists Fund of the National Natural Science Foundation of China(81400509);Corresponden-ce: Su Yuan, E-mail: maggie_suyuan@126.com

摘要/Abstract

摘要： 目的

探讨内质网应激（ERS）关键信号分子X盒结合蛋白1（XBP1）在牙周炎致病菌牙龈卟啉单胞菌（P. gingivalis）来源的脂多糖（LPS）刺激下对脂肪细胞胰岛素信号通路的影响。

方法

原代培养大鼠脂肪细胞，通过P. gingivalis-LPS（100 ng·mL^-1）刺激大鼠脂肪细胞4、8、12、24 h，蛋白质印迹法检测胰岛素信号通路胰岛素受体底物-1（IRS-1）、磷酸化磷脂酰肌醇依赖性激酶-1（p-PDK-1）和磷酸化蛋白激酶B（p-AKT）的蛋白表达；再将空载过表达慢病毒（pLVX-NC1）、过表达XBP1慢病毒（pLVX-XBP1）、空载干扰慢病毒（pLVX-NC2）、干扰XBP1慢病毒（pLVX-XBP1-RNAi）分别转染脂肪细胞，通过P. gingivalis-LPS刺激转染后的大鼠脂肪细胞，蛋白质印迹法检测胰岛素信号通路的蛋白表达。

结果

在P. gingivalis-LPS刺激下，大鼠脂肪细胞的胰岛素信号通路蛋白IRS-1、p-PDK-1、p-AKT表达呈下降趋势，差异有统计学意义（P<0.05）；pLVX-XBP1组的IRS-1、p-PDK-1、p-AKT蛋白表达在P. gingivalis-LPS刺激8、12 h后整体高于pLVX-NC1组，差异有统计学意义（P<0.05）；pLVX-XBP1-RNAi组在P. gingivalis-LPS刺激4、8、12、24 h后IRS-1、p-PDK-1和p-AKT蛋白表达整体低于pLVX-NC2组，差异有统计学意义（P<0.05）。

结论

P. gingivalis-LPS通过XBP1调控脂肪细胞胰岛素信号通路。

关键词: X盒结合蛋白1, 胰岛素信号通路, 脂肪细胞, 内质网应激, 牙周炎, 糖尿病

Abstract: Objective

This study aims to investigate the effect of X-box binding protein 1 (XBP1), a key signal molecule of ERS, on the insulin signaling pathway in adipocytes stimulated by Porphyromonas gingivalis (P. gingivalis)-lipopolysaccharide (LPS), a pathogenic bacterium of periodontitis.

Methods

Primary cultured rat adipocytes were stimulated by P. gingivalis-LPS (100 ng·mL^-1) for 4, 8, 12, and 24 h. The protein expression levels of insulin receptor substrate-1 (IRS-1), phosphoinositide dependent protein kinase (p-PDK-1), and protein kinase B (p-AKT-1) in the insulin signaling pathway were detected by Western blot analysis. pLVX-NC1, pLVX-XBP1, pLVX-NC2, and pLVX-XBP1-RNAi were transfected into adipocytes, respectively. The transfected rat adipocytes were stimulated by P. gingivalis-LPS, and the protein expression of the insulin signaling pathway was detected by Western blot.

Results

The Western Blot showed decreased protein expression of the insulin signaling pathway in rat adipocytes stimulated with P. gingivalis-LPS compared with the control, and the difference was statistically significant (P<0.05). The protein expression levels of IRS-1, p-PDK-1, and p-AKT in the rat adipocytes of pLVX-XBP1 were significantly higher than those in pLVX-NC1 at 8 and 12 h after P. gingivalis-LPS stimulation (P<0.05). The protein expression levels of IRS-1, p-PDK-1, and p-AKT in the rat adipocytes of pLVX-XBP1-RNAi were significantly lower than those in pLVX-NC2 at 4, 8, 12, and 24 h after P. gingivalis-LPS stimulation (P<0.05).

Conclusion

P. gingivalis-LPS regulates the insulin signaling pathway in adipocytes th-rough XBP1.

Key words: X-box binding protein 1, insulin signal pathway, adipocytes, endoplasmic reticulum stress, perio-dontitis, diabetes

中图分类号:

Q 257

陆佳艺, 伍倩琪, 陈伊燕, 叶蕾蕾, 苏媛. 牙龈卟啉单胞菌来源的脂多糖通过X盒结合蛋白1调控脂肪细胞胰岛素信号通路的机制研究[J]. 华西口腔医学杂志, 2022, 40(2): 148-154.

Lu Jiayi, Wu Qianqi, Chen Yiyan, Ye Leilei, Su Yuan. Mechanism of Porphyromonas gingivalis-lipopolysaccharide in regulating the insulin signaling pathway in adipocytes via X-box binding protein 1[J]. West China Journal of Stomatology, 2022, 40(2): 148-154.

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牙龈卟啉单胞菌来源的脂多糖通过X盒结合蛋白1调控脂肪细胞胰岛素信号通路的机制研究

Mechanism of Porphyromonas gingivalis-lipopolysaccharide in regulating the insulin signaling pathway in adipocytes via X-box binding protein 1

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