华西口腔医学杂志

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人唇腺和腮腺CCL28表达比较

刘雪1 姜淑敏2 唐伟3 姚丽霞1 王更如1 姜广水1   

  1. 1.山东大学口腔医学院口腔生物医学实验室, 山东济南250012;2.山东省荣军医院口腔科, 山东济南250013; 3.山东大学医学院医学微生物学教研室, 山东济南250012
  • 收稿日期:2009-10-25 修回日期:2009-10-25 出版日期:2009-10-20 发布日期:2009-10-20
  • 通讯作者: 姜广水,Tel:0531-88382940
  • 作者简介: 刘雪(1984—),女,山东人,硕士
  • 基金资助:

    国家自然科学基金资助项目(30171010和30371323);山东省自然科学基金资助项目(Y2006c124)

Comparison of CCL28 in human labial glands and parotids

LIU Xue1, JIANG Shu-min2, TANG Wei3, YAO Li-xia1, WANG Geng-ru1, JIANG Guang-shui1   

  1. 1. Oral Biological Laboratory, School of Stomatology, Shandong University, Jinan 250012, China; 2. Dept. of Stomatology, Provincial Veteran Hospital of Shandong, Jinan 250013, China; 3. Dept. of Microbiology, School of Medicine, Shandong University, Jinan 250012, China
  • Received:2009-10-25 Revised:2009-10-25 Online:2009-10-20 Published:2009-10-20
  • Contact: JIANG Guang-shui,Tel:0531-88382940

摘要:

目的比较人小涎腺与大涎腺CCL28的表达差异,以期探讨CCL28在小涎腺IgA分泌中的作用。方法应用实时荧光定量PCR技术,分别检测13例健康成人唇腺和8例健康成人腮腺标本中CCL28 mRNA表达的相对量;然后用SPSS 10.0统计软件对数据进行秩和检验。结果CCL28 mRNA在人的唇腺中有丰富的表达,并且唇腺中的CCL28 mRNA的表达量高于腮腺(P<0.01)。结论唇腺中CCL28表达量显著高于腮腺,说明小涎腺分泌高浓度IgA可能与CCL28高表达有关。

关键词: 唇腺, 腮腺, 实时荧光定量PCR

Abstract:

Objective To compare the expression of CCL28 in minor and major salivary glands and clarify the role it plays in IgA secreting by minor salivary glands in oral cavity. Methods Labial gland and parotid samples were analyzed with real-time fluorescent quantitative PCR assay for CCL28 mRNA. Rank-sum test was used for data analysis using SPSS 10.0 software package. Results CCL28 mRNA was abundantly expressed in labial glands of healthy adults. Its expression was higher than that in parotids(P<0.01). Conclusion The resuits of this article suggest that the expression level of CCL28 in labial glands is remarkably higher than that in parotids, which reminds us that the high concentration of IgA in minor salivary glands may be associated with their high expression of CCL28.

Key words: labial gland, parotid, real-time fluorescent quantitative PCR