West China Journal of Stomatology ›› 2019, Vol. 37 ›› Issue (6): 577-582.doi: 10.7518/hxkq.2019.06.002

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Protein kinase D1 regulates the growth and metabolism of oral squamous carcinoma cells in tumor microenvironment

Wang Liwei,Yu Yu,Chen Jiao,Feng Yun,Cui Bomiao,Li Xiaoying,Wang Jingnan,Chen Hongli,Zhang Ping()   

  1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2019-03-24 Revised:2019-08-02 Online:2019-12-01 Published:2019-11-27
  • Contact: Ping Zhang E-mail:964267060@qq.com
  • Supported by:
    The National Natural Science Foundation of China(81372892)


Objective To observe the effect of protein kinase D1 (PKD1) on the growth and metabolism of oral squamous cell carcinoma HSC-4 cells and related molecular mechanisms in the tumor microenvironment.Methods HSC-4 cell lines were transfected with shRNA plasmids. Three groups (Wild, control-shRNA, and PKD1-shRNA) were cultured under acidic or hypoxic environment for a certain time. Western blot was used to detect the expression of autophagy-related and glycolytic-related proteins. The proliferation changes were detected by CCK-8 kits.Results The PKD1-knockdown HSC-4 cell line was established. PKD1 silencing increased autophagy activity. Under hypoxic and acidic conditions, the PKD1-knockdown HSC-4 cells showed lower proliferation than the parental cells. PKD1-knockdown also decreased the expression of hypoxia induciblefactor 1α (HIF-1α) and pyruvate kinase M2 (PKM2).Conclusion Under hypoxic and acidic conditions, PKD1 gene silencing can increase apoptotic autophagy activity. Downregulated PKD1 gene expression can reduce the glycolysis of oral squamous cell carcinoma cells and inhibit tumor cell proliferation. This study revealed the important role of PKD1 in the metabolism and growth of oral squamous cell carcinoma, making it a possible target for the treatment of oral squamous cell carcinoma.

Key words: protein kinase D1, oral squamous cell carcinoma, tumor microenvironment, glycolysis metabolism, cell proliferation

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