Experimental study of demineralized dentin matrix on osteoinduction and related cells identification

doi:10.7518/hxkq.2018.01.007

Abstract

Abstract:

Objective The aim of this study was to explore the theoretical framework of cells and the forms of osteogenesis in the mechanism by which demineralized dentin matrix (DDM) induces osteogenesis. Methods A total of 24 New Zealand rabbits were used in this study. A total of 4 erector spinae bags were created in each animal. A total of 3 erector spinae bags were implanted with DDM by random selection, whereas the remaining one erector spinae bag was not implanted with DDM. The rabbits were sacrificed after 1, 2, 3, 4, 8, 12, 16, and 20 weeks, and the samples were obtained. The samples were examined by hematoxylin-eosin (HE), tartrate-resistant acid phosphatase (TRAP), and immunohistochemical staining to identify the mesenchymal stem cells, osteoblasts, chondrocytes, and osteoclasts. Results The results of HE staining showed that in the third week, cartilage- and bone-like matrices, as well as the osteoblast-like cells, were observed. The results of immunohisto-chemical staining showed that the expressions of CD44, alkaline phosphatase (ALP), and collagen Ⅱ were statistically significant (P<0.05). Conclusion DDM has good histocompatibility and osteoinduction. In addition, induced ectopic osteogenesis mode mainly occurs in the endochondral bone.

Key words: demineralized dentin matrix, osteoinduction, osteoblast, mesenchymal stem cells, chondrocytes

CLC Number:

Shengyin Yang, Ping Chen, Jibo Bao, Yixin Ding, Jinyang Zou, Zhigang Xie. Experimental study of demineralized dentin matrix on osteoinduction and related cells identification[J]. West China Journal of Stomatology, 2018, 36(1): 33-38.

Figures/Tables 8

Fig 1

Fig 2

Fig 3

Fig 4

Fig 5

Fig 6

Fig 7

Fig 8

References 19

[1]	Urist MR.Bone: formation by autoinduction[J]. Science, 1965, 150(3698):893-899.
[2]	Bang G, Urist MR.Bone induction in excavation chambers in matrix of decalcified dentin[J]. Arch Surg, 1967, 94(6):
	781-789.
[3]	Somerman MJ, Prince CW, Sauk JJ, et al.Mechanism of fibroblast attachment to bone extracellular matrix: role of a 44 kilodalton bone phosphoprotein[J]. J Bone Miner Res, 1987, 2(3):259-265.
[4]	Yeomans JD, Urist MR.Bone induction by decalcified den-tine implanted into oral, osseous and muscle tissues[J]. Arch Oral Biol, 1967, 12(8):999-1008.
[5]	杨胜银, 谢志刚. 脱矿牙本质基质的应用研究[J]. 临床口腔医学杂志, 2014, 30(3):189-191.
	Yang SY, Xie ZG.Applied study of demineralized dentin matrix[J]. J Clin Stomatol, 2014, 30(3):189-191.
[6]	胡蕴玉. 现代骨科基础与临床[M]. 北京: 人民卫生出版社, 2006:3-29, 108-136, 727-736, 742-773.
	Hu YY.Contemporary orthopaedics: basic science and cli-nical pratice[M]. Beijing: People’s Medical Publishing House, 2006:3-29, 108-136, 727-736, 742-773.
[7]	Reddi AH, Huggins C.Biochemical sequences in the trans-formation of normal fibroblasts in adolescent rats[J]. Proc Nat Acad Sci U S A, 1972, 69(6):1601-1605.
[8]	Reddi A, Anderson WA.Collagenous bone matrix-induced endochondral ossification hemopoiesis[J]. J Cell Biol, 1976, 69(3):557-572.
[9]	Reddi AH.Cell biology and biochemistry of endochondral bone development[J]. Coll Relat Res, 1981, 1(2):209-226.
[10]	Atala A, Lanza R, Thomson JA, et al.Principles of regene-rative medicine (second edition)[M].Oxford:Elsevier, 2011: 253-262, 285-304, 347-364.
[11]	Urist MR, Lietze A, Mizutani H, et al.A bovine low mole-cular weight bone morphogenetic protein (BMP) fraction [J]. Clin Orthop Relat Res, 1982(162):219-232.
[12]	Urist MR, Delange RJ, Finerman G.Bone cell differentia-tion and growth factors[J].Science, 1983, 220(4598):680-686
[13]	Bergsma JE, Rozema FR, Bos RR, et al. Biocompatibility study of as-polymerized poly (L-lactide)in rats using a cage implant system[J].J Biomed Mater Res, 1995, 29(2):173-179
[14]	Mizutani H, Mera K, Ueda M, et al.A study of the bone morphogenetic protein derived from bovine demineralized dentin matrix[J]. Nagoya J Med Sci, 1996, 59(1/2):37-47.
[15]	Gomes MF, Banzi EC, Destro MF, et al. Homogenous de-mineralized dentin matrix for application in cranioplasty of rabbits with alloxan-induced diabetes: histomorphometric analysis[J].Int J Oral Maxillofac Implants, 2007,22(6):939--949
[16]	Yagihashi K, Miyazawa K, Togari K, et al.Demineralized dentin matrix acts as a scaffold for repair of articular carti-lage defects[J]. Calcif Tissue Int, 2009, 84(3):210-220.
[17]	Stoeger T, Proetzel G, Welzel H, et al.In situ gene expression analysis during BMP2-induced ectopic bone formation in mice shows simultaneous endochondral and intramembra-nous ossification[J]. Growth Factor, 2003, 20(4):197-210.
[18]	李瑞, 王青山.生物材料生物相容性的评价方法和发展趋势[J]. 中国组织工程研究与临床康复, 2011, 15(29):5471-5474
	5474.
19	Li R, Wang QS.Evaluated methods and developmental trend of biocompatibility of biomaterials[J]. J Clin Rehabilitat Tissue Eng Res, 2011, 15(29):5471-5474.
[19]	Moore GE.Foreign body carcinogenesis[J]. Cancer, 1991, 67(11):2731-2732.