West China Journal of Stomatology ›› 2020, Vol. 38 ›› Issue (2): 133-138.doi: 10.7518/hxkq.2020.02.004

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Study on expression of LIM domain only protein 1 in SD rat oral buccal mucosa carcinogenesis induced by 4-nitroquinoline N-oxide

Yu Li1,2,3, Liu Xuqian1,2, Chen Yuhe1,2, Chen Xiao4, Nie Minhai1,2()   

  1. 1. Oral & Maxillofacial Reconstruction Laboratory, Southwest Medical University, Luzhou 646000, China
    2. Dept. of Periodontal Mucosal Disease, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, China
    3. Dept. of Oral Diagnosis and Medicine, Division of Oral Pathobiological Science, Graduate School of Dental Medicine, Hokkaido University, Hokkaido 060-0808, Japan
    4. Dept. of Orthodontics, Mianyang Stomatological Hospital, Mianyang 621000, China
  • Received:2019-07-07 Revised:2020-01-15 Online:2020-04-01 Published:2020-04-15
  • Contact: Minhai Nie E-mail:nieminhai@126.com
  • Supported by:
    Sichuan Medical Association Scientific Research Project(S17071);Sichuan Science and Technology Department Project(2018JY040)


Objective This work aimed to determine the expression changes in LIM domain only protein 1 (LMO1) in gene transcription and protein levels during oral squamous cell carcinoma (OSCC) development. Methods The tissues in this study were taken from our team’s previous animal model building, and we performed hematoxylin-eosin (HE) staining on 49 cases. The pathological classification of the experiment group was determined on the basis of the abnormal epithelial hyperplasia degree. The expression part of LMO1 was determined by immunohistochemistry staining. The mRNA and protein LMO1 expression levels in five groups were detected by real-time fluorescent quantitative of nucleotide polymer chain reaction (RT-qPCR) and Western blot, respectively. Results HE staining determined 7 cases of the control group, 6 cases of mild epithelial dysplasia, 11 cases of moderate epithelial dysplasia, 9 cases of severe epithelial dysplasia, and 16 cases of OSCC. Immunohistochemistry results: LMO1 expression was localized in the cytoplasm, and the positive expression rates of the protein LMO1 in the control and experiment groups were 14.3% for normal buccal mucosal tissue, 33.3% for mild epithelial dysplasia, 81.8% for moderate epithelial dysplasia, 88.9% for severe epithelial dysplasia, and 93.8% for OSCC. RT-qPCR results: mRNA expression was lowest in the control group and highest in the OSCC group, the difference between the mild dysplasia and control groups was not significant (P>0.05). Pairwise comparison among other groups showed statistically significant differences (P<0.05). Western blot results: with the aggravation of the pathological degree, the protein LMO1 expression level increased gradually. The OSCC group expressed the highest LMO1 expression level. Conclusion The oral mucosa carcinogenesis models showed abnormal the mRNA and protein LMO1 expression levels, and the mRNA and protein expression levels were positively correlated with the degree of abnormal proliferation.

Key words: LIM domain only protein 1, oral squamous cell carcinoma, epithelial dysplasia

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